Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Conformational ensembles of the magnesium channel CorA reveal structural basis for channel gating.
Journal, issue, pages	Proc Natl Acad Sci U S A, Vol. 123, Issue 8, Page e2512532123, Year 2026
Publish date	Feb 24, 2026
Authors	Satchal K Erramilli / Kamil Nosol / Krzysztof Pietrzak-Lichwa / Nicolaus Schmandt / Tian Li / Piotr Tokarz / Jingkai Hou / Minglei Zhao / Eduardo Perozo / Anthony A Kossiakoff /
PubMed Abstract	In prokaryotes, CorA is the primary influx pathway for magnesium, a critical divalent cation in cellular physiology and biochemistry. Mechanistic studies show that homopentameric CorA is regulated ...In prokaryotes, CorA is the primary influx pathway for magnesium, a critical divalent cation in cellular physiology and biochemistry. Mechanistic studies show that homopentameric CorA is regulated through an intracellular [Mg]-dependent negative feedback loop, involving the asymmetric participation of individual subunits. To understand the connection between asymmetry and activation, we used single-particle cryo-EM to solve sixteen structures of nanodisc-reconstituted CorA. We utilized conformation-specific synthetic antibodies to stabilize subtle but significant conformational differences in the cryo-EM structures. Our results demonstrate that CorA exists as a set of conformational ensembles, where population size inversely correlates with intracellular Mg concentration. These ensembles include channels with a variety of pore conformations, both constricted and dilated, suggesting a spectrum of active CorA functional states. The ensembles connect asymmetric structural transitions in the cytoplasmic domain with conformational changes in the permeation pathway via an electrostatic network, ultimately controlling channel-gating events. We believe that these results establish a framework for understanding magnesium homeostasis in prokaryotic systems.
External links	Proc Natl Acad Sci U S A / PubMed:41701836
Methods	EM (single particle)
Resolution	3.0 - 4.1 Å
Structure data	41384 8tmc EMDB-41384, PDB-8tmc: Cryo-EM structure of CorA in complex with conformation-specific synthetic antibody C12 and 20 mM MgCl2, State MG20-2 Method: EM (single particle) / Resolution: 3.3 Å 41385 8tmd EMDB-41385, PDB-8tmd: Cryo-EM structure of CorA in complex with conformation-specific synthetic antibody C18 and 100 uM MgCl2, State MG0.1-1A Method: EM (single particle) / Resolution: 3.0 Å 41386 8tme EMDB-41386, PDB-8tme: Cryo-EM structure of CorA in complex with conformation-specific synthetic antibody C18 and 100 uM MgCl2, State MG0.1-1B Method: EM (single particle) / Resolution: 3.1 Å 41387 8tmf EMDB-41387, PDB-8tmf: Cryo-EM structure of CorA in complex with conformation-specific synthetic antibody C18 and 100 uM MgCl2, State MG0.1-1C Method: EM (single particle) / Resolution: 3.4 Å 41388 8tmg EMDB-41388, PDB-8tmg: Cryo-EM structure of CorA in complex with conformation-specific synthetic antibody C18 and 100 uM MgCl2, State MG0.1-2A Method: EM (single particle) / Resolution: 3.0 Å 41389 8tmh EMDB-41389, PDB-8tmh: Cryo-EM structure of CorA in complex with conformation-specific synthetic antibody C18 and 100 uM MgCl2, State MG0.1-2B Method: EM (single particle) / Resolution: 3.1 Å 41390 8tmi EMDB-41390, PDB-8tmi: Cryo-EM structure of CorA in complex with conformation-specific synthetic antibody C18 and 100 uM MgCl2, State MG0.1-2C Method: EM (single particle) / Resolution: 3.3 Å 41391 8tmj EMDB-41391, PDB-8tmj: Cryo-EM structure of CorA in complex with conformation-specific synthetic antibody C18 and 100 uM MgCl2, State MG0.1-2D Method: EM (single particle) / Resolution: 3.2 Å 41392 8tmk EMDB-41392, PDB-8tmk: Cryo-EM structure of magnesium depleted CorA in complex with conformation-specific synthetic antibody C18, State MGD-2C Method: EM (single particle) / Resolution: 3.4 Å 41393 8tml EMDB-41393, PDB-8tml: Cryo-EM structure of magnesium depleted CorA in complex with conformation-specific synthetic antibody C18, State MGD-2B Method: EM (single particle) / Resolution: 3.4 Å 41394 8tmm EMDB-41394, PDB-8tmm: Cryo-EM structure of magnesium depleted CorA in complex with conformation-specific synthetic antibody C18, State MGD-2A Method: EM (single particle) / Resolution: 3.4 Å 41395 8tmn EMDB-41395, PDB-8tmn: Cryo-EM structure of magnesium depleted CorA in complex with conformation-specific synthetic antibody C18, State MGD-1D Method: EM (single particle) / Resolution: 3.3 Å 41396 8tmo EMDB-41396, PDB-8tmo: Cryo-EM structure of magnesium depleted CorA in complex with conformation-specific synthetic antibody C18, State MGD-1C Method: EM (single particle) / Resolution: 3.1 Å 41397 8tmp EMDB-41397, PDB-8tmp: Cryo-EM structure of magnesium depleted CorA in complex with conformation-specific synthetic antibody C18, State MGD-1B Method: EM (single particle) / Resolution: 3.2 Å 41398 8tmq EMDB-41398, PDB-8tmq: Cryo-EM structure of magnesium depleted CorA in complex with conformation-specific synthetic antibody C18, State MGD-1A Method: EM (single particle) / Resolution: 3.1 Å EMDB-41404: Local cryo-EM refinement map (Chain C N-terminal domain) for magnesium depleted CorA in complex with conformation-specific synthetic antibody C18, State MGD-1D Method: EM (single particle) / Resolution: 4.1 Å EMDB-41405: Local cryo-EM refinement map (Chain D N-terminal domain) for magnesium depleted CorA in complex with conformation-specific synthetic antibody C18, State MGD-1D Method: EM (single particle) / Resolution: 4.1 Å EMDB-41406: Consensus cryo-EM refinement map for magnesium depleted CorA in complex with conformation-specific synthetic antibody C18, State MGD-1D Method: EM (single particle) / Resolution: 3.3 Å EMDB-41412: Local cryo-EM refinement map (Chain C N-terminal domain) for magnesium depleted CorA in complex with conformation-specific synthetic antibody C18, State MGD-1C Method: EM (single particle) / Resolution: 4.0 Å EMDB-41413: Local cryo-EM refinement map (Chain D N-terminal domain) for magnesium depleted CorA in complex with conformation-specific synthetic antibody C18, State MGD-1C Method: EM (single particle) / Resolution: 3.6 Å EMDB-41414: Consensus cryo-EM refinement map for magnesium depleted CorA in complex with conformation-specific synthetic antibody C18, State MGD-1C Method: EM (single particle) / Resolution: 3.1 Å EMDB-41419: Local cryo-EM refinement map (Chain D N-terminal domain) for CorA in complex with conformation-specific synthetic antibody C18 and 100 uM MgCl2, State MG0.1-1B Method: EM (single particle) / Resolution: 3.7 Å EMDB-41420: Local cryo-EM refinement map (Chain C N-terminal domain) for CorA in complex with conformation-specific synthetic antibody C18 and 100 uM MgCl2, State MG0.1-1B Method: EM (single particle) / Resolution: 3.7 Å EMDB-41421: Consensus cryo-EM refinement map for CorA in complex with conformation-specific synthetic antibody C18 and 100 uM MgCl2, State MG0.1-1B Method: EM (single particle) / Resolution: 3.1 Å
Chemicals	ChemComp-MG: Unknown entry
Source	thermotoga maritima (bacteria) homo sapiens (human)
Keywords	MEMBRANE PROTEIN / Ion Channel / Magnesium Channel