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Structure paper

TitleTuning Chemical DNA Ligation within DNA Crystals and Protein-DNA Cocrystals.
Journal, issue, pagesAcs Nanosci Au, Vol. 4, Page 338-348, Year 2024
Publish dateJul 20, 2023 (structure data deposition date)
AuthorsOrun, A.R. / Slaughter, C.K. / Shields, E.T. / Vajapayajula, A. / Jones, S. / Shrestha, R. / Snow, C.D.
External linksAcs Nanosci Au / PubMed:39430379
MethodsX-ray diffraction
Resolution2.45 - 3.9 Å
Structure data

PDB-8tip:
Isoreticular, interpenetrating co-crystal of Replication Initiator Protein REPE54 and symmetrical expanded duplex (31mer) containing the cognate REPE54 sequence and an additional G-C rich sequence with blunt ends and 5' terminal phosphates.
Method: X-RAY DIFFRACTION / Resolution: 2.79 Å

PDB-8tiq:
Isoreticular, interpenetrating co-crystal of Replication Initiator Protein REPE54 and symmetrical expanded duplex (31mer) containing the cognate REPE54 sequence and an additional G-C rich sequence with blunt ends and 3' terminal phosphates.
Method: X-RAY DIFFRACTION / Resolution: 2.45 Å

PDB-8tir:
Isoreticular, interpenetrating co-crystal of Replication Initiator Protein REPE54 and symmetrical expanded duplex (31mer) containing the cognate REPE54 sequence and an additional G-C rich sequence with 1 sticky bases and 5' terminal phosphates.
Method: X-RAY DIFFRACTION / Resolution: 3.11 Å

PDB-8tis:
Isoreticular, interpenetrating co-crystal of Replication Initiator Protein REPE54 and symmetrical expanded duplex (31mer) containing the cognate REPE54 sequence and an additional G-C rich sequence with 1 sticky bases and 3' terminal phosphates.
Method: X-RAY DIFFRACTION / Resolution: 2.54 Å

PDB-8tit:
Isoreticular, interpenetrating co-crystal of Replication Initiator Protein REPE54 and symmetrical expanded duplex (31mer) containing the cognate REPE54 sequence and an additional G-C rich sequence with 2 sticky base overhangs and no terminal phosphates.
Method: X-RAY DIFFRACTION / Resolution: 2.84 Å

PDB-8tiu:
Isoreticular, interpenetrating co-crystal of Replication Initiator Protein REPE54 and symmetrical expanded duplex (31mer) containing the cognate REPE54 sequence and an additional G-C rich sequence with 2 sticky base overhangs and 3' terminal phosphates.
Method: X-RAY DIFFRACTION / Resolution: 3.9 Å

PDB-8tiv:
Isoreticular, interpenetrating co-crystal of Replication Initiator Protein REPE54 and symmetrical expanded duplex (31mer) containing the cognate REPE54 sequence and an additional G-C rich sequence with blunt ends and 5' terminal phosphates and crosslinked with EDC.
Method: X-RAY DIFFRACTION / Resolution: 3.35 Å

PDB-8tiw:
Isoreticular, interpenetrating co-crystal of Replication Initiator Protein REPE54 and symmetrical expanded duplex (31mer) containing the cognate REPE54 sequence and an additional G-C rich sequence with blunt ends and 3' terminal phosphates and crosslinked with EDC.
Method: X-RAY DIFFRACTION / Resolution: 3.11 Å

PDB-8tix:
Isoreticular, interpenetrating co-crystal of Replication Initiator Protein REPE54 and symmetrical expanded duplex (31mer) containing the cognate REPE54 sequence and an additional G-C rich sequence with 1 sticky bases and 5' terminal phosphates and crosslinked with EDC.
Method: X-RAY DIFFRACTION / Resolution: 2.91 Å

PDB-8tiy:
Isoreticular, interpenetrating co-crystal of Replication Initiator Protein REPE54 and symmetrical expanded duplex (31mer) containing the cognate REPE54 sequence and an additional G-C rich sequence with 1 sticky bases and 3' terminal phosphates and crosslinked with EDC.
Method: X-RAY DIFFRACTION / Resolution: 3.11 Å

PDB-8tiz:
Isoreticular, interpenetrating co-crystal of Replication Initiator Protein REPE54 and symmetrical expanded duplex (31mer) containing the cognate REPE54 sequence and an additional G-C rich sequence with 2 sticky base overhangs and no terminal phosphates and crosslinked with EDC.
Method: X-RAY DIFFRACTION / Resolution: 3.11 Å

PDB-8tj0:
Isoreticular, interpenetrating co-crystal of Replication Initiator Protein REPE54 and symmetrical expanded duplex (31mer) containing the cognate REPE54 sequence and an additional G-C rich sequence with 2 sticky base overhangs and 5' terminal phosphates and crosslinked with EDC.
Method: X-RAY DIFFRACTION / Resolution: 3.24 Å

PDB-8tj1:
Isoreticular, interpenetrating co-crystal of Replication Initiator Protein REPE54 and symmetrical expanded duplex (31mer) containing the cognate REPE54 sequence and an additional G-C rich sequence with 2 sticky base overhangs and 3' terminal phosphates and crosslinked with EDC.
Method: X-RAY DIFFRACTION / Resolution: 3.15 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-HOH:
WATER

Source
  • escherichia coli k-12 (bacteria)
  • escherichia coli (E. coli)
KeywordsDNA BINDING PROTEIN/DNA / Replication Initiator RepE Complex Co-Crystal / DNA BINDING PROTEIN-DNA complex / DNA BINDING PROTEIN

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