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| タイトル | Structure determination of a DNA crystal by MicroED. |
|---|---|
| ジャーナル・号・ページ | Structure, Vol. 31, Issue 12, Page 1499-11503.e2, Year 2023 |
| 掲載日 | 2023年12月7日 |
著者 | Alison Haymaker / Andrey A Bardin / Tamir Gonen / Michael W Martynowycz / Brent L Nannenga / ![]() |
| PubMed 要旨 | Microcrystal electron diffraction (MicroED) is a powerful tool for determining high-resolution structures of microcrystals from a diverse array of biomolecular, chemical, and material samples. In ...Microcrystal electron diffraction (MicroED) is a powerful tool for determining high-resolution structures of microcrystals from a diverse array of biomolecular, chemical, and material samples. In this study, we apply MicroED to DNA crystals, which have not been previously analyzed using this technique. We utilized the d(CGCGCG) DNA duplex as a model sample and employed cryo-FIB milling to create thin lamella for diffraction data collection. The MicroED data collection and subsequent processing resulted in a 1.10 Å resolution structure of the d(CGCGCG) DNA, demonstrating the successful application of cryo-FIB milling and MicroED to the investigation of nucleic acid crystals. |
リンク | Structure / PubMed:37541248 / PubMed Central |
| 手法 | EM (電子線結晶学) |
| 解像度 | 1.1 Å |
| 構造データ | ![]() PDB-8skw: |
| 化合物 | ![]() ChemComp-SPM: ![]() ChemComp-HOH: |
| 由来 |
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キーワード | DNA / Z-DNA |
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