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TitleStabilization of the hexasome intermediate during histone exchange by yeast SWR1 complex.
Journal, issue, pagesMol Cell, Year 2024
Publish dateAug 28, 2024
AuthorsAdam S B Jalal / Paul Girvan / Eugene Y D Chua / Lexin Liu / Shijie Wang / Elizabeth A McCormack / Michael T Skehan / Carol L Knight / David S Rueda / Dale B Wigley /
PubMed AbstractThe yeast SWR1 complex catalyzes the exchange of histone H2A/H2B dimers in nucleosomes with Htz1/H2B dimers. We use cryoelectron microscopy to determine the structure of an enzyme-bound hexasome ...The yeast SWR1 complex catalyzes the exchange of histone H2A/H2B dimers in nucleosomes with Htz1/H2B dimers. We use cryoelectron microscopy to determine the structure of an enzyme-bound hexasome intermediate in the reaction pathway of histone exchange, in which an H2A/H2B dimer has been extracted from a nucleosome prior to the insertion of a dimer comprising Htz1/H2B. The structure reveals a key role for the Swc5 subunit in stabilizing the unwrapping of DNA from the histone core of the hexasome. By engineering a crosslink between an Htz1/H2B dimer and its chaperone protein Chz1, we show that this blocks histone exchange by SWR1 but allows the incoming chaperone-dimer complex to insert into the hexasome. We use this reagent to trap an SWR1/hexasome complex with an incoming Htz1/H2B dimer that shows how the reaction progresses to the next step. Taken together the structures reveal insights into the mechanism of histone exchange by SWR1 complex.
External linksMol Cell / PubMed:39226902
MethodsEM (single particle)
Resolution3.5 - 4.4 Å
Structure data

18764

8qyv

EMDB-18764, PDB-8qyv:
SWR1-hexasome complex
Method: EM (single particle) / Resolution: 3.5 Å

18769

8qz0

EMDB-18769, PDB-8qz0:
SWR1-hexasome-dimer complex
Method: EM (single particle) / Resolution: 3.8 Å

50297

9fbw

EMDB-50297, PDB-9fbw:
SWR1 lacking Swc5 subunit in complex with hexasome
Method: EM (single particle) / Resolution: 4.4 Å

Chemicals

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM

ChemComp-BEF:
BERYLLIUM TRIFLUORIDE ION

ChemComp-MG:
Unknown entry

ChemComp-ZN:
Unknown entry

Source
  • saccharomyces cerevisiae s288c (yeast)
  • synthetic construct (others)
KeywordsDNA BINDING PROTEIN / Chromatin remodelling complex / hexasome / hexasome-dimer complex

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