EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structure and activation mechanism of the Makes caterpillars floppy 1 toxin.
ジャーナル・号・ページ	Nat Commun, Vol. 14, Issue 1, Page 8226, Year 2023
掲載日	2023年12月12日
著者	Alexander Belyy / Philipp Heilen / Philine Hagel / Oliver Hofnagel / Stefan Raunser /
PubMed 要旨	The bacterial Makes caterpillars floppy 1 (Mcf1) toxin promotes apoptosis in insects, leading to loss of body turgor and death. The molecular mechanism underlying Mcf1 intoxication is poorly ...The bacterial Makes caterpillars floppy 1 (Mcf1) toxin promotes apoptosis in insects, leading to loss of body turgor and death. The molecular mechanism underlying Mcf1 intoxication is poorly understood. Here, we present the cryo-EM structure of Mcf1 from Photorhabdus luminescens, revealing a seahorse-like shape with a head and tail. While the three head domains contain two effectors, as well as an activator-binding domain (ABD) and an autoprotease, the tail consists of two putative translocation and three putative receptor-binding domains. Rearrangement of the tail moves the C-terminus away from the ABD and allows binding of the host cell ADP-ribosylation factor 3, inducing conformational changes that position the cleavage site closer to the protease. This distinct activation mechanism that is based on a hook-loop interaction results in three autocleavage reactions and the release of two toxic effectors. Unexpectedly, the BH3-like domain containing ABD is not an active effector. Our findings allow us to understand key steps of Mcf1 intoxication at the molecular level.
リンク	Nat Commun / PubMed:38086871 / PubMed Central
手法	EM (単粒子)
解像度	3.55 - 4.3 Å
構造データ	17435 8p50 EMDB-17435, PDB-8p50: Photorhabdus luminescens Makes caterpillars floppy (Mcf) toxin with the C-terminal deletion in complex with Arf3 手法: EM (単粒子) / 解像度: 4.04 Å 17436 8p51 EMDB-17436, PDB-8p51: Photorhabdus luminescens Makes caterpillars floppy (Mcf) toxin with the C-terminal deletion 手法: EM (単粒子) / 解像度: 3.55 Å EMDB-17437: Photorhabdus luminescens Makes caterpillars floppy (Mcf) toxin, the central region of the toxin 手法: EM (単粒子) / 解像度: 3.6 Å EMDB-17438: Photorhabdus luminescens Makes caterpillars floppy (Mcf) toxin, the tail region of the toxin 手法: EM (単粒子) / 解像度: 3.6 Å EMDB-17439: Photorhabdus luminescens Makes caterpillars floppy (Mcf) toxin, the head of the toxin 手法: EM (単粒子) / 解像度: 4.1 Å 17440 8p52 EMDB-17440, PDB-8p52: Photorhabdus luminescens Makes caterpillars floppy (Mcf) toxin 手法: EM (単粒子) / 解像度: 3.6 Å EMDB-17450: Photorhabdus luminescens Makes caterpillars floppy (Mcf) toxin, the low-resolution consensus map 手法: EM (単粒子) / 解像度: 4.3 Å
化合物	ChemComp-GTP: GUANOSINE-5'-TRIPHOSPHATE / GTP / GTP, エネルギー貯蔵分子YM / グアノシン三リン酸 ChemComp-MG*: Unknown entry / マグネシウムジカチオン
由来	photorhabdus luminescens (バクテリア) homo sapiens (ヒト)
キーワード	TOXIN (毒素) / Bacterial toxin / Arf