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Structure paper

TitleStructural basis for activity switching in polymerases determining the fate of let-7 pre-miRNAs.
Journal, issue, pagesNat Struct Mol Biol, Vol. 31, Issue 9, Page 1426-1438, Year 2024
Publish dateJul 25, 2024
AuthorsGangshun Yi / Mingda Ye / Loic Carrique / Afaf El-Sagheer / Tom Brown / Chris J Norbury / Peijun Zhang / Robert J C Gilbert /
PubMed AbstractTumor-suppressor let-7 pre-microRNAs (miRNAs) are regulated by terminal uridylyltransferases TUT7 and TUT4 that either promote let-7 maturation by adding a single uridine nucleotide to the pre-miRNA ...Tumor-suppressor let-7 pre-microRNAs (miRNAs) are regulated by terminal uridylyltransferases TUT7 and TUT4 that either promote let-7 maturation by adding a single uridine nucleotide to the pre-miRNA 3' end or mark them for degradation by the addition of multiple uridines. Oligo-uridylation is increased in cells by enhanced TUT7/4 expression and especially by the RNA-binding pluripotency factor LIN28A. Using cryogenic electron microscopy, we captured high-resolution structures of active forms of TUT7 alone, of TUT7 plus pre-miRNA and of both TUT7 and TUT4 bound with pre-miRNA and LIN28A. Our structures reveal that pre-miRNAs engage the enzymes in fundamentally different ways depending on the presence of LIN28A, which clamps them onto the TUTs to enable processive 3' oligo-uridylation. This study reveals the molecular basis for mono- versus oligo-uridylation by TUT7/4, as determined by the presence of LIN28A, and thus their mechanism of action in the regulation of cell fate and in cancer.
External linksNat Struct Mol Biol / PubMed:39054354 / PubMed Central
MethodsEM (single particle)
Resolution3.65 - 4.0 Å
Structure data

16825

8oef

EMDB-16825, PDB-8oef:
Structure of human terminal uridylyltransferase 7 (hTUT7/ZCCHC6)
Method: EM (single particle) / Resolution: 4.0 Å

17084

8opp

EMDB-17084, PDB-8opp:
Structure of human terminal uridylyltransferase 7 (hTUT7/ZCCHC6) bound with pre-let7g miRNA and UTPalphaS
Method: EM (single particle) / Resolution: 3.76 Å

17086

8ops

EMDB-17086, PDB-8ops:
Human terminal uridylyltransferase 7 (TUT7/ZCCHC6) bound with pre-let7g miRNA and Lin28A - complex 1
Method: EM (single particle) / Resolution: 3.82 Å

17087

8opt

EMDB-17087, PDB-8opt:
Human terminal uridylyltransferase 7 (TUT7/ZCCHC6) bound with pre-let7g miRNA and Lin28A - complex 2
Method: EM (single particle) / Resolution: 3.65 Å

17164

8ost

EMDB-17164, PDB-8ost:
Structure of human terminal uridylyltransferase 4 (TUT4, ZCCHC11) in complex with pre-let7g miRNA and Lin28A
Method: EM (single particle) / Resolution: 3.69 Å

Chemicals

ChemComp-P5E:
[[(2~{R},3~{S},4~{R},5~{R})-5-[2,4-bis(oxidanylidene)pyrimidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-sulfanyl-phosphoryl] phosphono hydrogen phosphate

ChemComp-ZN:
Unknown entry

Source
  • homo sapiens (human)
KeywordsRNA / Polymerase / uridylation / RNA maturation and turnover control / RNA BINDING PROTEIN / CELL CYCLE

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