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Structure paper

TitleC-type inactivation and proton modulation mechanisms of the TASK3 channel.
Journal, issue, pagesProc Natl Acad Sci U S A, Vol. 121, Issue 17, Page e2320345121, Year 2024
Publish dateApr 23, 2024
AuthorsHuajian Lin / Junnan Li / Qiansen Zhang / Huaiyu Yang / Shanshuang Chen /
PubMed AbstractThe TWIK-related acid-sensitive K channel 3 (TASK3) belongs to the two-pore domain (K2P) potassium channel family, which regulates cell excitability by mediating a constitutive "leak" potassium ...The TWIK-related acid-sensitive K channel 3 (TASK3) belongs to the two-pore domain (K2P) potassium channel family, which regulates cell excitability by mediating a constitutive "leak" potassium efflux in the nervous system. Extracellular acidification inhibits TASK3 channel, but the molecular mechanism by which channel inactivation is coupled to pH decrease remains unclear. Here, we report the cryo-electron microscopy structures of human TASK3 at neutral and acidic pH. Structural comparison revealed selectivity filter (SF) rearrangements upon acidification, characteristic of C-type inactivation, but with a unique structural basis. The extracellular mouth of the SF was prominently dilated and simultaneously blocked by a hydrophobic gate. His98 protonation shifted the conformational equilibrium between the conductive and C-type inactivated SF toward the latter by engaging a cation-π interaction with Trp78, consistent with molecular dynamics simulations and electrophysiological experiments. Our work illustrated how TASK3 is gated in response to extracellular pH change and implies how physiological stimuli might directly modulate the C-type gating of K2P channels.
External linksProc Natl Acad Sci U S A / PubMed:38630723 / PubMed Central
MethodsEM (single particle)
Resolution3.0 - 3.68 Å
Structure data

36793

8k1j

EMDB-36793, PDB-8k1j:
Human TWIK-related acid-sensitive potassium channel TASK3 at pH 7.4,200 mM KCl
Method: EM (single particle) / Resolution: 3.0 Å

36799

8k1q

EMDB-36799, PDB-8k1q:
Human TWIK-related acid-sensitive potassium channel TASK3 at pH 6.0, 5 mM KCl and 135 mM NaCl
Method: EM (single particle) / Resolution: 3.68 Å

36805

8k1v

EMDB-36805, PDB-8k1v:
Human TWIK-related acid-sensitive potassium channel TASK3 at pH 7.4, 5 mM KCl and 135 mM NaCl
Method: EM (single particle) / Resolution: 3.48 Å

36806

8k1z

EMDB-36806, PDB-8k1z:
Human TWIK-related acid-sensitive potassium channel TASK3 at pH 6.0, 200 mM KCl
Method: EM (single particle) / Resolution: 3.41 Å

Chemicals

ChemComp-Y01:
CHOLESTEROL HEMISUCCINATE

ChemComp-K:
Unknown entry

ChemComp-HOH:
WATER

Source
  • homo sapiens (human)
KeywordsMEMBRANE PROTEIN / Acid-sensitive / potassium ion channel / C-type / gating mechanism

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