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Structure paper

TitleAnchoring a Structurally Editable Proximal Cofactor-like Module to Construct an Artificial Dual-center Peroxygenase.
Journal, issue, pagesAngew. Chem. Int. Ed. Engl., Vol. 62, Page e202311259-e202311259, Year 2023
Publish dateJun 6, 2022 (structure data deposition date)
AuthorsQin, X. / Jiang, Y. / Yao, F. / Chen, J. / Kong, F. / Zhao, P. / Jin, L. / Cong, Z.
External linksAngew. Chem. Int. Ed. Engl. / PubMed:37713467
MethodsX-ray diffraction
Resolution1.75 - 2.01 Å
Structure data

PDB-7y0t:
Crystal structure of the P450 BM3 heme domain mutant F87A in complex with N-imidazolyl-hexanoyl-L-phenylalanyl-L-phenylalanine
Method: X-RAY DIFFRACTION / Resolution: 1.89 Å

PDB-8hon:
Crystal structure of the P450 BM3 heme domain mutant F87A in complex with Im-C6-Tyr-Tyr
Method: X-RAY DIFFRACTION / Resolution: 2.01 Å

PDB-8hop:
Crystal structure of the P450 BM3 heme domain mutant F87A in complex with Im-C6-Nap-Tyr
Method: X-RAY DIFFRACTION / Resolution: 1.86 Å

PDB-8hoq:
Crystal structure of the P450 BM3 heme domain mutant F87A in complex with Im-C6-Phe(4CF3)-Tyr
Method: X-RAY DIFFRACTION / Resolution: 1.94 Å

PDB-8hor:
Crystal structure of the P450 BM3 heme domain mutant F87A in complex with Im-C6-Phe(4CH3)-Tyr
Method: X-RAY DIFFRACTION / Resolution: 1.95 Å

PDB-8hos:
Crystal structure of the P450 BM3 heme domain mutant F87A in complex with Im-C6-Phe(4NO2)-Tyr
Method: X-RAY DIFFRACTION / Resolution: 1.82 Å

PDB-8hou:
Crystal structure of the P450 BM3 heme domain mutant F87A-T268V in complex with Im-N-C4-Phe-Phe
Method: X-RAY DIFFRACTION / Resolution: 1.75 Å

Chemicals

ChemComp-HEM:
PROTOPORPHYRIN IX CONTAINING FE

ChemComp-HOH:
WATER

Source
  • priestia megaterium nbrc 15308 = atcc 14581 (bacteria)
  • synthetic construct (others)
  • priestia megaterium (bacteria)
KeywordsOXIDOREDUCTASE / Dual-functional small molecule / P450 heme domain / Complex

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