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TitleStructure-based rational design of Bacillus licheniformis L-asparaginase with low/no D-asparaginase activity for a safer enzyme
Journal, issue, pagesTo Be Published
Publish dateOct 10, 2022 (structure data deposition date)
AuthorsLu, F. / Wang, W. / Chi, H. / Ran, T.
External linksSearch PubMed
MethodsX-ray diffraction
Resolution1.6 - 2.06 Å
Structure data

PDB-8h44:
Blasnase-P55N
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

PDB-8h45:
Blasnase-T13A/P55N
Method: X-RAY DIFFRACTION / Resolution: 1.97 Å

PDB-8h46:
Blasnase-T13A/P55N with L-asn
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

PDB-8h47:
Blasnase-T13A/P55F
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-8h48:
Blasnase-T13A/P55F with L-asn
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

PDB-8h49:
Blasnase-M57P
Method: X-RAY DIFFRACTION / Resolution: 2 Å

PDB-8h4a:
Blasnase-T13A/M57P
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-8h4b:
Blasnase-T13A/M57P with L-asn
Method: X-RAY DIFFRACTION / Resolution: 2 Å

PDB-8h4c:
Blasnase-T13A/M57P
Method: X-RAY DIFFRACTION / Resolution: 1.6 Å

PDB-8h4d:
Blasnase-T13A/M57N
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-8h4e:
Blasnase-T13A/P55N with D-asn
Method: X-RAY DIFFRACTION / Resolution: 2.06 Å

PDB-8h4f:
Blasnase-T13A/P55F with D-asn
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-8h4g:
Blasnase-T13A/M57N
Method: X-RAY DIFFRACTION / Resolution: 1.81 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-FMT:
FORMIC ACID

ChemComp-HOH:
WATER

ChemComp-ASN:
ASPARAGINE

ChemComp-GOL:
GLYCEROL

ChemComp-DSG:
D-ASPARAGINE

Source
  • bacillus paralicheniformis (bacteria)
KeywordsHYDROLASE / Mutant

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