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TitleFunctionalized graphene grids with various charges for single-particle cryo-EM.
Journal, issue, pagesNat Commun, Vol. 13, Issue 1, Page 6718, Year 2022
Publish dateNov 7, 2022
AuthorsYe Lu / Nan Liu / Yongbo Liu / Liming Zheng / Junhao Yang / Jia Wang / Xia Jia / Qinru Zi / Hailin Peng / Yu Rao / Hong-Wei Wang /
PubMed AbstractA major hurdle for single particle cryo-EM in structural determination lies in the specimen preparation impaired by the air-water interface (AWI) and preferential particle-orientation problems. In ...A major hurdle for single particle cryo-EM in structural determination lies in the specimen preparation impaired by the air-water interface (AWI) and preferential particle-orientation problems. In this work, we develop functionalized graphene grids with various charges via a dediazoniation reaction for cryo-EM specimen preparation. The graphene grids are paraffin-assistant fabricated, which appear with less contaminations compared with those produced by polymer transfer method. By applying onto three different types of macromolecules, we demonstrate that the high-yield charged graphene grids bring macromolecules away from the AWI and enable adjustable particle-orientation distribution for more robust single particle cryo-EM structural determination.
External linksNat Commun / PubMed:36344519 / PubMed Central
MethodsEM (single particle)
Resolution3.2 Å
Structure data

PDB-8h2h:
Cryo-EM structure of a Group II Intron Complexed with its Reverse Transcriptase
Method: ELECTRON MICROSCOPY / Resolution: 3.2 Å

Source
  • lactococcus lactis (lactic acid bacteria)
KeywordsRNA BINDING PROTEIN/RNA / intron / RNA BINDING PROTEIN-RNA COMPLEX

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