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Structure paper

TitleStructures of a FtsZ single protofilament and a double-helical tube in complex with a monobody.
Journal, issue, pagesNat Commun, Vol. 14, Issue 1, Page 4073, Year 2023
Publish dateJul 10, 2023
AuthorsJunso Fujita / Hiroshi Amesaka / Takuya Yoshizawa / Kota Hibino / Natsuki Kamimura / Natsuko Kuroda / Takamoto Konishi / Yuki Kato / Mizuho Hara / Tsuyoshi Inoue / Keiichi Namba / Shun-Ichi Tanaka / Hiroyoshi Matsumura /
PubMed AbstractFtsZ polymerizes into protofilaments to form the Z-ring that acts as a scaffold for accessory proteins during cell division. Structures of FtsZ have been previously solved, but detailed mechanistic ...FtsZ polymerizes into protofilaments to form the Z-ring that acts as a scaffold for accessory proteins during cell division. Structures of FtsZ have been previously solved, but detailed mechanistic insights are lacking. Here, we determine the cryoEM structure of a single protofilament of FtsZ from Klebsiella pneumoniae (KpFtsZ) in a polymerization-preferred conformation. We also develop a monobody (Mb) that binds to KpFtsZ and FtsZ from Escherichia coli without affecting their GTPase activity. Crystal structures of the FtsZ-Mb complexes reveal the Mb binding mode, while addition of Mb in vivo inhibits cell division. A cryoEM structure of a double-helical tube of KpFtsZ-Mb at 2.7 Å resolution shows two parallel protofilaments. Our present study highlights the physiological roles of the conformational changes of FtsZ in treadmilling that regulate cell division.
External linksNat Commun / PubMed:37429870 / PubMed Central
MethodsEM (helical sym.) / X-ray diffraction
Resolution1.84 - 3.03 Å
Structure data

34429

8h1o

EMDB-34429, PDB-8h1o:
Cryo-EM structure of KpFtsZ-monobody double helical tube
Method: EM (helical sym.) / Resolution: 2.67 Å

35344

8ibn

EMDB-35344, PDB-8ibn:
Cryo-EM structure of KpFtsZ single filament
Method: EM (helical sym.) / Resolution: 3.03 Å

PDB-8gzv:
Klebsiella pneumoniae FtsZ complexed with monobody (P212121)
Method: X-RAY DIFFRACTION / Resolution: 2.2 Å

PDB-8gzw:
Klebsiella pneumoniae FtsZ complexed with monobody (P21)
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

PDB-8gzx:
Escherichia coli FtsZ complexed with monobody (P212121)
Method: X-RAY DIFFRACTION / Resolution: 1.84 Å

PDB-8gzy:
Escherichia coli FtsZ complexed with monobody (P21)
Method: X-RAY DIFFRACTION / Resolution: 2.6 Å

Chemicals

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM

ChemComp-HOH:
WATER

ChemComp-G2P:
PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER / GMP-CPP, energy-carrying molecule analogue*YM

ChemComp-K:
Unknown entry

Source
  • klebsiella pneumoniae (bacteria)
  • homo sapiens (human)
  • escherichia coli (E. coli)
KeywordsCELL CYCLE / Cell division / Monobody / GTPase / HYDROLASE / bacterial cell division / divisome / FtsZ / tubulin

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