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-Structure paper
| タイトル | An SI3-σ arch stabilizes cyanobacteria transcription initiation complex. |
|---|---|
| ジャーナル・号・ページ | Proc Natl Acad Sci U S A, Vol. 120, Issue 16, Page e2219290120, Year 2023 |
| 掲載日 | 2023年4月18日 |
著者 | Liqiang Shen / Giorgio Lai / Linlin You / Jing Shi / Xiaoxian Wu / Maria Puiu / Zhanxi Gu / Yu Feng / Yulia Yuzenkova / Yu Zhang / ![]() |
| PubMed 要旨 | Multisubunit RNA polymerases (RNAPs) associate with initiation factors (σ in bacteria) to start transcription. The σ factors are responsible for recognizing and unwinding promoter DNA in all ...Multisubunit RNA polymerases (RNAPs) associate with initiation factors (σ in bacteria) to start transcription. The σ factors are responsible for recognizing and unwinding promoter DNA in all bacterial RNAPs. Here, we report two cryo-EM structures of cyanobacterial transcription initiation complexes at near-atomic resolutions. The structures show that cyanobacterial RNAP forms an "SI3-σ" arch interaction between domain 2 of σ (σ) and sequence insertion 3 (SI3) in the mobile catalytic domain Trigger Loop (TL). The "SI3-σ" arch facilitates transcription initiation from promoters of different classes through sealing the main cleft and thereby stabilizing the RNAP-promoter DNA open complex. Disruption of the "SI3-σ" arch disturbs cyanobacteria growth and stress response. Our study reports the structure of cyanobacterial RNAP and a unique mechanism for its transcription initiation. Our data suggest functional plasticity of SI3 and provide the foundation for further research into cyanobacterial and chloroplast transcription. |
リンク | Proc Natl Acad Sci U S A / PubMed:37036976 / PubMed Central |
| 手法 | EM (単粒子) / X線回折 |
| 解像度 | 1.552 - 3.13 Å |
| 構造データ | EMDB-34397, PDB-8gzg: EMDB-34398, PDB-8gzh: ![]() PDB-8h02: |
| 化合物 | ![]() ChemComp-MG: ![]() ChemComp-ZN: ![]() ChemComp-CTP: ![]() ChemComp-HOH: |
| 由来 |
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キーワード | TRANSCRIPTION/DNA/RNA / Cryo-EM / Synechocystis sp. PCC 6803 / Syn6803 / Transcription initiation / RPitc / Transcription / Cyanobacteria / TRANSCRIPTION-DNA-RNA complex / CTP-bound RPitc / RNA polymerase / SI3 |
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