EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Assembly of the Tn7 targeting complex by a regulated stepwise process.
ジャーナル・号・ページ	Mol Cell, Vol. 84, Issue 12, Page 2368-22381.e6, Year 2024
掲載日	2024年6月20日
著者	Yao Shen / Shreya S Krishnan / Michael T Petassi / Mark A Hancock / Joseph E Peters / Alba Guarné /
PubMed 要旨	The Tn7 family of transposons is notable for its highly regulated integration mechanisms, including programmable RNA-guided transposition. The targeting pathways rely on dedicated target selection ...The Tn7 family of transposons is notable for its highly regulated integration mechanisms, including programmable RNA-guided transposition. The targeting pathways rely on dedicated target selection proteins from the TniQ family and the AAA+ adaptor TnsC to recruit and activate the transposase at specific target sites. Here, we report the cryoelectron microscopy (cryo-EM) structures of TnsC bound to the TniQ domain of TnsD from prototypical Tn7 and unveil key regulatory steps stemming from unique behaviors of ATP- versus ADP-bound TnsC. We show that TnsD recruits ADP-bound dimers of TnsC and acts as an exchange factor to release one protomer with exchange to ATP. This loading process explains how TnsC assembles a heptameric ring unidirectionally from the target site. This unique loading process results in functionally distinct TnsC protomers within the ring, providing a checkpoint for target immunity and explaining how insertions at programmed sites precisely occur in a specific orientation across Tn7 elements.
リンク	Mol Cell / PubMed:38834067 / PubMed Central
手法	EM (単粒子)
解像度	3.32 - 3.88 Å
構造データ	40218 8glu EMDB-40218, PDB-8glu: CryoEM structure of TnsC(1-503) bound to TnsD(1-318) from E.coli Tn7 手法: EM (単粒子) / 解像度: 3.57 Å 40221 8glw EMDB-40221, PDB-8glw: CryoEM structure of the TnsC(1-503)-TnsD(1-318)-DNA complex in a 7:2:1 stoichiometry from E. coli Tn7 手法: EM (単粒子) / 解像度: 3.51 Å 40222 8glx EMDB-40222, PDB-8glx: CryoEM structure of the TnsC(1-503)-TnsD(1-318)-DNA complex in a 6:2:1 stoichiometry from E. coli Tn7 手法: EM (単粒子) / 解像度: 3.88 Å 43138 8vcj EMDB-43138, PDB-8vcj: CryoEM structure of the TnsC(1-503)-TnsD(1-318)-DNA complex in a 7:2:1 stoichiometry from E. coli Tn7 bound to ATPgS and ADP 手法: EM (単粒子) / 解像度: 3.32 Å 43140 8vct EMDB-43140, PDB-8vct: CyoEM structure of the TnsC(1-503)-TnsD(1-318)-DNA complex in a 6:2:1 stoichiometry from E. coli Tn7 bound to ATPgS and ADP 手法: EM (単粒子) / 解像度: 3.83 Å
化合物	ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ZN: Unknown entry ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM ChemComp-AGS: PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER / ATP-γ-S / ATP-gamma-S, エネルギー貯蔵分子類似体*YM
由来	escherichia coli (大腸菌)
キーワード	DNA BINDING PROTEIN / Transposon / AAA+ ATPase / Oligomer / Complex / DNA BINDING PROTEIN/DNA / DNA BINDING PROTEIN-DNA complex / DNA-binding protein DNA complex