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TitleHigh-throughput reprogramming of an NRPS condensation domain.
Journal, issue, pagesNat Chem Biol, Vol. 20, Issue 6, Page 761-769, Year 2024
Publish dateFeb 2, 2024
AuthorsInes B Folger / Natália F Frota / Angelos Pistofidis / David L Niquille / Douglas A Hansen / T Martin Schmeing / Donald Hilvert /
PubMed AbstractEngineered biosynthetic assembly lines could revolutionize the sustainable production of bioactive natural product analogs. Although yeast display is a proven, powerful tool for altering the ...Engineered biosynthetic assembly lines could revolutionize the sustainable production of bioactive natural product analogs. Although yeast display is a proven, powerful tool for altering the substrate specificity of gatekeeper adenylation domains in nonribosomal peptide synthetases (NRPSs), comparable strategies for other components of these megaenzymes have not been described. Here we report a high-throughput approach for engineering condensation (C) domains responsible for peptide elongation. We show that a 120-kDa NRPS module, displayed in functional form on yeast, can productively interact with an upstream module, provided in solution, to produce amide products tethered to the yeast surface. Using this system to screen a large C-domain library, we reprogrammed a surfactin synthetase module to accept a fatty acid donor, increasing catalytic efficiency for this noncanonical substrate >40-fold. Because C domains can function as selectivity filters in NRPSs, this methodology should facilitate the precision engineering of these molecular assembly lines.
External linksNat Chem Biol / PubMed:38308044 / PubMed Central
MethodsX-ray diffraction
Resolution1.58 - 2.4 Å
Structure data

PDB-8f7f:
The condensation domain of surfactin A synthetase C in space group P43212
Method: X-RAY DIFFRACTION / Resolution: 1.62 Å

PDB-8f7g:
The condensation domain of surfactin A synthetase C in space group P212121
Method: X-RAY DIFFRACTION / Resolution: 2.4 Å

PDB-8f7h:
The condensation domain of surfactin A synthetase C variant 18b in space group P212121
Method: X-RAY DIFFRACTION / Resolution: 1.93 Å

PDB-8f7i:
The condensation domain of surfactin A synthetase C variant 18b in space group P43212
Method: X-RAY DIFFRACTION / Resolution: 1.58 Å

Chemicals

ChemComp-GOL:
GLYCEROL

ChemComp-HOH:
WATER

Source
  • bacillus subtilis (bacteria)
KeywordsBIOSYNTHETIC PROTEIN / NRPS / C domain / SrfA-C

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