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Structure paper

TitlePYK-SubstitutionOME: an integrated database containing allosteric coupling, ligand affinity and mutational, structural, pathological, bioinformatic and computational information about pyruvate kinase isozymes.
Journal, issue, pagesDatabase (Oxford), Vol. 2023, Year 2023
Publish dateNov 15, 2022 (structure data deposition date)
AuthorsSwint-Kruse, L. / Dougherty, L.L. / Page, B. / Wu, T. / O'Neil, P.T. / Prasannan, C.B. / Timmons, C. / Tang, Q. / Parente, D.J. / Sreenivasan, S. ...Swint-Kruse, L. / Dougherty, L.L. / Page, B. / Wu, T. / O'Neil, P.T. / Prasannan, C.B. / Timmons, C. / Tang, Q. / Parente, D.J. / Sreenivasan, S. / Holyoak, T. / Fenton, A.W.
External linksDatabase (Oxford) / PubMed:37171062
MethodsX-ray diffraction
Resolution2.15 - 2.41 Å
Structure data

PDB-8f5t:
Rabbit muscle pyruvate kinase in complex with sodium and magnesium
Method: X-RAY DIFFRACTION / Resolution: 2.41 Å

PDB-8f5u:
Rabbit muscle pyruvate kinase in complex with magnesium, potassium and pyruvate
Method: X-RAY DIFFRACTION / Resolution: 2.3 Å

PDB-8f6m:
Complex of Rabbit muscle pyruvate kinase with ADP and the phosphonate analogue of PEP mimicking the Michaelis complex.
Method: X-RAY DIFFRACTION / Resolution: 2.15 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-NA:
Unknown entry

ChemComp-TRS:
2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL / pH buffer*YM

ChemComp-GOL:
GLYCEROL

ChemComp-HOH:
WATER

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-K:
Unknown entry

ChemComp-PYR:
PYRUVIC ACID

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM

ChemComp-SIN:
SUCCINIC ACID

ChemComp-MN:
Unknown entry

ChemComp-ALA:
ALANINE

ChemComp-GZ3:
(E)-2-METHYL-3-PHOSPHONOACRYLATE

Source
  • oryctolagus cuniculus (rabbit)
KeywordsCYTOSOLIC PROTEIN / glycolysis / metabolic kinase / central metabolism

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