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TitleMechanism of actin filament branch formation by Arp2/3 complex revealed by a high-resolution cryo-EM structureof the branch junction.
Journal, issue, pagesProc Natl Acad Sci U S A, Vol. 119, Issue 49, Page e2206722119, Year 2022
Publish dateDec 6, 2022
AuthorsSteven Z Chou / Moon Chatterjee / Thomas D Pollard /
PubMed AbstractWe reconstructed the structure of actin filament branch junctions formed by fission yeast Arp2/3 complex at 3.5 Å resolution from images collected by electron cryo-microscopy. During specimen ...We reconstructed the structure of actin filament branch junctions formed by fission yeast Arp2/3 complex at 3.5 Å resolution from images collected by electron cryo-microscopy. During specimen preparation, all of the actin subunits and Arp3 hydrolyzed their bound adenosine triphosphate (ATP) and dissociated the γ-phosphate, but Arp2 retained the γ-phosphate. Binding tightly to the side of the mother filament and nucleating the daughter filament growing as a branch requires Arp2/3 complex to undergo a dramatic conformational change where two blocks of structure rotate relative to each other about 25° to align Arp2 and Arp3 as the first two subunits in the branch. During branch formation, Arp2/3 complex acquires more than 8,000 Å of new buried surface, accounting for the stability of the branch. Inactive Arp2/3 complex binds only transiently to the side of an actin filament, because its conformation allows only a subset of the interactions found in the branch junction.
External linksProc Natl Acad Sci U S A / PubMed:36442092 / PubMed Central
MethodsEM (single particle)
Resolution3.5 Å
Structure data

27962

8e9b

EMDB-27962, PDB-8e9b:
Cryo-EM structure of S. pombe Arp2/3 complex in the branch junction
Method: EM (single particle) / Resolution: 3.5 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM

Source
  • schizosaccharomyces pombe (fission yeast)
  • gallus gallus (chicken)
  • Fission yeast (fission yeast)
  • chicken (chicken)
KeywordsSTRUCTURAL PROTEIN / Arp2-3 complex / branch juction / polymerization

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