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Title | A co-transcriptional ribosome assembly checkpoint controls nascent large ribosomal subunit maturation. |
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Journal, issue, pages | Nat Struct Mol Biol, Vol. 30, Issue 5, Page 594-599, Year 2023 |
Publish date | Apr 10, 2023 |
![]() | Zahra A Sanghai / Rafal Piwowarczyk / Arnaud Vanden Broeck / Sebastian Klinge / ![]() |
PubMed Abstract | During transcription of eukaryotic ribosomal DNA in the nucleolus, assembly checkpoints exist that guarantee the formation of stable precursors of small and large ribosomal subunits. While the ...During transcription of eukaryotic ribosomal DNA in the nucleolus, assembly checkpoints exist that guarantee the formation of stable precursors of small and large ribosomal subunits. While the formation of an early large subunit assembly checkpoint precedes the separation of small and large subunit maturation, its mechanism of action and function remain unknown. Here, we report the cryo-electron microscopy structure of the yeast co-transcriptional large ribosomal subunit assembly intermediate that serves as a checkpoint. The structure provides the mechanistic basis for how quality-control pathways are established through co-transcriptional ribosome assembly factors, that structurally interrogate, remodel and, together with ribosomal proteins, cooperatively stabilize correctly folded pre-ribosomal RNA. Our findings thus provide a molecular explanation for quality control during eukaryotic ribosome assembly in the nucleolus. |
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Methods | EM (single particle) |
Resolution | 3.69 - 4.0 Å |
Structure data | ![]() EMDB-27910: Yeast nucleolar Noc2-Noc3 RNP pre-60S assembly intermediate (state E) EMDB-27919, PDB-8e5t: |
Chemicals | ![]() ChemComp-MG: ![]() ChemComp-ZN: |
Source |
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![]() | RIBOSOME / Co-transcriptional pre-60S / ribosome biogenesis / early check-point intermediate |