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TitleFast viral dynamics revealed by microsecond time-resolved cryo-EM.
Journal, issue, pagesNat Commun, Vol. 14, Issue 1, Page 5649, Year 2023
Publish dateSep 13, 2023
AuthorsOliver F Harder / Sarah V Barrass / Marcel Drabbels / Ulrich J Lorenz /
PubMed AbstractObserving proteins as they perform their tasks has largely remained elusive, which has left our understanding of protein function fundamentally incomplete. To enable such observations, we have ...Observing proteins as they perform their tasks has largely remained elusive, which has left our understanding of protein function fundamentally incomplete. To enable such observations, we have recently proposed a technique that improves the time resolution of cryo-electron microscopy (cryo-EM) to microseconds. Here, we demonstrate that microsecond time-resolved cryo-EM enables observations of fast protein dynamics. We use our approach to elucidate the mechanics of the capsid of cowpea chlorotic mottle virus (CCMV), whose large-amplitude motions play a crucial role in the viral life cycle. We observe that a pH jump causes the extended configuration of the capsid to contract on the microsecond timescale. While this is a concerted process, the motions of the capsid proteins involve different timescales, leading to a curved reaction path. It is difficult to conceive how such a detailed picture of the dynamics could have been obtained with any other method, which highlights the potential of our technique. Crucially, our experiments pave the way for microsecond time-resolved cryo-EM to be applied to a broad range of protein dynamics that previously could not have been observed. This promises to fundamentally advance our understanding of protein function.
External linksNat Commun / PubMed:37704664 / PubMed Central
MethodsEM (single particle)
Resolution1.64 - 8.0 Å
Structure data

16400

8c38

EMDB-16400, PDB-8c38:
Contracted cowpea chlorotic mottle virus
Method: EM (single particle) / Resolution: 1.64 Å

16790

8cpy

EMDB-16790, PDB-8cpy:
Extended cowpea chlorotic mottle virus
Method: EM (single particle) / Resolution: 3.9 Å

EMDB-16798: Partially contracted cowpea chlorotic mottle virus
Method: EM (single particle) / Resolution: 8.0 Å

EMDB-16857: Extended cowpea chlorotic mottle virus at pH 7.6 in plunge-frozen vitrified ice
Method: EM (single particle) / Resolution: 4.1 Å

EMDB-16858: Extended cowpea chlorotic mottle virus at pH 7.6 in laser-melted and revitrified ice
Method: EM (single particle) / Resolution: 4.3 Å

Source
  • cowpea chlorotic mottle virus
KeywordsVIRUS / Icosahedral / contracted / extended

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