EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Modulation of translational decoding by mA modification of mRNA.
ジャーナル・号・ページ	Nat Commun, Vol. 14, Issue 1, Page 4784, Year 2023
掲載日	2023年8月8日
著者	Sakshi Jain / Lukasz Koziej / Panagiotis Poulis / Igor Kaczmarczyk / Monika Gaik / Michal Rawski / Namit Ranjan / Sebastian Glatt / Marina V Rodnina /
PubMed 要旨	N-methyladenosine (mA) is an abundant, dynamic mRNA modification that regulates key steps of cellular mRNA metabolism. mA in the mRNA coding regions inhibits translation elongation. Here, we show how ...N-methyladenosine (mA) is an abundant, dynamic mRNA modification that regulates key steps of cellular mRNA metabolism. mA in the mRNA coding regions inhibits translation elongation. Here, we show how mA modulates decoding in the bacterial translation system using a combination of rapid kinetics, smFRET and single-particle cryo-EM. We show that, while the modification does not impair the initial binding of aminoacyl-tRNA to the ribosome, in the presence of mA fewer ribosomes complete the decoding process due to the lower stability of the complexes and enhanced tRNA drop-off. The mRNA codon adopts a π-stacked codon conformation that is remodeled upon aminoacyl-tRNA binding. mA does not exclude canonical codon-anticodon geometry, but favors alternative more dynamic conformations that are rejected by the ribosome. These results highlight how modifications outside the Watson-Crick edge can still interfere with codon-anticodon base pairing and complex recognition by the ribosome, thereby modulating the translational efficiency of modified mRNAs.
リンク	Nat Commun / PubMed:37553384 / PubMed Central
手法	EM (単粒子)
解像度	2.04 - 2.88 Å
構造データ	16015 8bf7 EMDB-16015, PDB-8bf7: Elongating E. coli 70S ribosome containing deacylated tRNA(iMet) in the P-site and AAA mRNA codon with cognate dipeptidyl-tRNA(Lys) in the A-site 手法: EM (単粒子) / 解像度: 2.33 Å 16029 8bge EMDB-16029, PDB-8bge: Elongating E. coli 70S ribosome containing acylated tRNA(iMet) in the P-site and AAm6A mRNA codon in the A-site after uncompleted di-peptide formation 手法: EM (単粒子) / 解像度: 2.11 Å 16031 8bgh EMDB-16031, PDB-8bgh: Elongating E. coli 70S ribosome containing acylated tRNA(iMet) in the P-site and AAA mRNA codon in the A-site after uncompleted di-peptide formation 手法: EM (単粒子) / 解像度: 2.88 Å 16047 8bh4 EMDB-16047, PDB-8bh4: Elongating E. coli 70S ribosome containing deacylated tRNA(iMet) in the P-site and AAm6A mRNA codon with cognate dipeptidyl-tRNA(Lys) in the A-site 手法: EM (単粒子) / 解像度: 2.62 Å 16057 8bhj EMDB-16057, PDB-8bhj: Elongating E. coli 70S ribosome containing deacylated tRNA(iMet) in the P-site and Am6AA mRNA codon with cognate dipeptidyl-tRNA(Lys) in the A-site 手法: EM (単粒子) / 解像度: 2.81 Å 16059 8bhl EMDB-16059, PDB-8bhl: Elongating E. coli 70S ribosome containing acylated tRNA(iMet) in the P-site and Am6AA mRNA codon in the A-site after uncompleted di-peptide formation 手法: EM (単粒子) / 解像度: 2.21 Å 16062 8bhn EMDB-16062, PDB-8bhn: Elongating E. coli 70S ribosome containing deacylated tRNA(iMet) in the P-site and m6AAA mRNA codon with cognate dipeptidyl-tRNA(Lys) in the A-site 手法: EM (単粒子) / 解像度: 2.85 Å 16065 8bhp EMDB-16065, PDB-8bhp: Elongating E. coli 70S ribosome containing acylated tRNA(iMet) in the P-site and m6AAA mRNA codon in the A-site after uncompleted di-peptide formation 手法: EM (単粒子) / 解像度: 2.37 Å 16081 8bil EMDB-16081, PDB-8bil: Initiation complex of the E. coli 70S ribosome with mRNA containing AAA codon in the A-site. 手法: EM (単粒子) / 解像度: 2.04 Å 16082 8bim EMDB-16082, PDB-8bim: Initiation complex of the E. coli 70S ribosome with mRNA containing AAm6A codon in the A-site 手法: EM (単粒子) / 解像度: 2.04 Å
化合物	ChemComp-FME: N-FORMYLMETHIONINE / N-ホルミルメチオニン
由来	escherichia coli k-12 (大腸菌) synthetic construct (人工物)
キーワード	TRANSLATION / m6A / mRNA modification / tRNA recognition