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TitleA general method for the development of multicolor biosensors with large dynamic ranges.
Journal, issue, pagesNat. Chem. Biol., Vol. 19, Page 1147-1157, Year 2023
Publish dateSep 27, 2022 (structure data deposition date)
AuthorsHellweg, L. / Edenhofer, A. / Barck, L. / Huppertz, M.C. / Frei, M.S. / Tarnawski, M. / Bergner, A. / Koch, B. / Johnsson, K. / Hiblot, J.
External linksNat. Chem. Biol. / PubMed:37291200
MethodsX-ray diffraction
Resolution1.5 - 2 Å
Structure data

PDB-8b6r:
X-ray structure of the haloalkane dehalogenase HaloTag7 labeled with a chloroalkane Cyanine3 fluorophore substrate
Method: X-RAY DIFFRACTION / Resolution: 1.5 Å

PDB-8b6s:
X-ray structure of the haloalkane dehalogenase HaloTag7 fusion to the green fluorescent protein GFP (ChemoG1) labeled with a chloroalkane tetramethylrhodamine fluorophore substrate
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

PDB-8b6t:
X-ray structure of the interface optimized haloalkane dehalogenase HaloTag7 fusion to the green fluorescent protein GFP (ChemoG5-TMR) labeled with a chloroalkane tetramethylrhodamine fluorophore substrate
Method: X-RAY DIFFRACTION / Resolution: 2 Å

Chemicals

ChemComp-PJI:
~{N}-[2-[2-(6-chloranylhexoxy)ethoxy]ethyl]-6-[3,3-dimethyl-2-[(~{E})-3-(1,3,3-trimethylindol-2-ylidene)prop-1-enyl]indol-1-ium-1-yl]hexanamide

ChemComp-CL:
Unknown entry

ChemComp-GOL:
GLYCEROL

ChemComp-MG:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-OEH:
[9-[2-carboxy-5-[2-[2-(6-chloranylhexoxy)ethoxy]ethylcarbamoyl]phenyl]-6-(dimethylamino)xanthen-3-ylidene]-dimethyl-azanium

Source
  • rhodococcus sp. (bacteria)
KeywordsHYDROLASE / haloalkane dehalogenase / HaloTag / HaloTag7 / Self-Labeling Protein / fluorophore / cyanine 3 / Cy3 / green fluorescent protein / GFP / tetramethylerhodamine / TMR

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