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TitleCryo-EM structure of the agonist-bound Hsp90-XAP2-AHR cytosolic complex.
Journal, issue, pagesNat Commun, Vol. 13, Issue 1, Page 7010, Year 2022
Publish dateNov 16, 2022
AuthorsJakub Gruszczyk / Loïc Grandvuillemin / Josephine Lai-Kee-Him / Matteo Paloni / Christos G Savva / Pierre Germain / Marina Grimaldi / Abdelhay Boulahtouf / Hok-Sau Kwong / Julien Bous / Aurélie Ancelin / Cherine Bechara / Alessandro Barducci / Patrick Balaguer / William Bourguet /
PubMed AbstractThe aryl hydrocarbon receptor (AHR) is a ligand-dependent transcription factor that mediates a broad spectrum of (patho)physiological processes in response to numerous substances including ...The aryl hydrocarbon receptor (AHR) is a ligand-dependent transcription factor that mediates a broad spectrum of (patho)physiological processes in response to numerous substances including pollutants, natural products and metabolites. However, the scarcity of structural data precludes understanding of how AHR is activated by such diverse compounds. Our 2.85 Å structure of the human indirubin-bound AHR complex with the chaperone Hsp90 and the co-chaperone XAP2, reported herein, reveals a closed conformation Hsp90 dimer with AHR threaded through its lumen and XAP2 serving as a brace. Importantly, we disclose the long-awaited structure of the AHR PAS-B domain revealing a unique organisation of the ligand-binding pocket and the structural determinants of ligand-binding specificity and promiscuity of the receptor. By providing structural details of the molecular initiating event leading to AHR activation, our study rationalises almost forty years of biochemical data and provides a framework for future mechanistic studies and structure-guided drug design.
External linksNat Commun / PubMed:36385050 / PubMed Central
MethodsEM (single particle)
Resolution2.85 - 4.07 Å
Structure data

14971

7zub

EMDB-14971, PDB-7zub:
Cryo-EM structure of the indirubin-bound Hsp90-XAP2-AHR complex
Method: EM (single particle) / Resolution: 2.85 Å

EMDB-14972: Cryo-EM structure of the indirubin-bound Hsp90-XAP2-AHR complex (focused map).
Method: EM (single particle) / Resolution: 4.07 Å

Chemicals

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM / Adenosine diphosphate

ChemComp-MG:
Unknown entry

ChemComp-MOO:
MOLYBDATE ION / Molybdate

ChemComp-JY6:
(3~{Z})-3-(3-oxidanylidene-1~{H}-indol-2-ylidene)-1~{H}-indol-2-one / Indirubin

Source
  • homo sapiens (human)
KeywordsGENE REGULATION / complex / nuclear receptor / chemical pollutants / detoxification / cancer

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