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Structure paper

TitleVisualizing the DNA repair process by a photolyase at atomic resolution.
Journal, issue, pagesScience, Vol. 382, Page eadd7795-eadd7795, Year 2023
Publish dateJul 1, 2022 (structure data deposition date)
AuthorsMaestre-Reyna, M. / Wang, P.H. / Nango, E. / Hosokawa, Y. / Saft, M. / Furrer, A. / Yang, C.H. / Gusti Ngurah Putu, E.P. / Wu, W.J. / Emmerich, H.J. ...Maestre-Reyna, M. / Wang, P.H. / Nango, E. / Hosokawa, Y. / Saft, M. / Furrer, A. / Yang, C.H. / Gusti Ngurah Putu, E.P. / Wu, W.J. / Emmerich, H.J. / Caramello, N. / Franz-Badur, S. / Yang, C. / Engilberge, S. / Wranik, M. / Glover, H.L. / Weinert, T. / Wu, H.Y. / Lee, C.C. / Huang, W.C. / Huang, K.F. / Chang, Y.K. / Liao, J.H. / Weng, J.H. / Gad, W. / Chang, C.W. / Pang, A.H. / Yang, K.C. / Lin, W.T. / Chang, Y.C. / Gashi, D. / Beale, E. / Ozerov, D. / Nass, K. / Knopp, G. / Johnson, P.J.M. / Cirelli, C. / Milne, C. / Bacellar, C. / Sugahara, M. / Owada, S. / Joti, Y. / Yamashita, A. / Tanaka, R. / Tanaka, T. / Luo, F. / Tono, K. / Zarzycka, W. / Muller, P. / Alahmad, M.A. / Bezold, F. / Fuchs, V. / Gnau, P. / Kiontke, S. / Korf, L. / Reithofer, V. / Rosner, C.J. / Seiler, E.M. / Watad, M. / Werel, L. / Spadaccini, R. / Yamamoto, J. / Iwata, S. / Zhong, D. / Standfuss, J. / Royant, A. / Bessho, Y. / Essen, L.O. / Tsai, M.D.
External linksScience / PubMed:38033054
MethodsX-ray diffraction
Resolution1.95 - 2.75 Å
Structure data

PDB-7yc7:
Dark, fully reduced structure of the MmCPDII-DNA complex as produced at SwissFEL
Method: X-RAY DIFFRACTION / Resolution: 1.95 Å

PDB-7ycm:
TR-SFX MmCPDII-DNA complex: 100 ps snapshot. Includes 100ps, dark, and extrapolated structure factors
Method: X-RAY DIFFRACTION / Resolution: 2 Å

PDB-7ycp:
TR-SFX MmCPDII-DNA complex: 250 ps snapshot. Includes 250 ps, dark, and extrapolated structure factors
Method: X-RAY DIFFRACTION / Resolution: 2.08 Å

PDB-7ycr:
TR-SFX MmCPDII-DNA complex: 450 ps snapshot. Includes 450ps, dark, and extrapolated structure factors
Method: X-RAY DIFFRACTION / Resolution: 2.15 Å

PDB-7yd6:
TR-SFX MmCPDII-DNA complex: 650 ps snapshot. Includes 650ps, dark, and extrapolated structure factors
Method: X-RAY DIFFRACTION / Resolution: 2.15 Å

PDB-7yd7:
TR-SFX MmCPDII-DNA complex: 1 ns snapshot. Includes 1 ns, dark, and extrapolated structure factors
Method: X-RAY DIFFRACTION / Resolution: 2.25 Å

PDB-7yd8:
TR-SFX MmCPDII-DNA complex: 2 ns snapshot. Includes 2 ns, dark, and extrapolated structure factors
Method: X-RAY DIFFRACTION / Resolution: 2.15 Å

PDB-7ydz:
TR-SFX MmCPDII-DNA complex: dark state as collected in SACLA
Method: X-RAY DIFFRACTION / Resolution: 2.23 Å

PDB-7ye0:
DF-SFX MmCPDII-DNA complex: steady state oxidized complex
Method: X-RAY DIFFRACTION / Resolution: 2.75 Å

PDB-7yeb:
TR-SFX MmCPDII-DNA complex: 3.35 ns snapshot. Includes 3.35 ns, dark, and extrapolated structure factors
Method: X-RAY DIFFRACTION / Resolution: 2.2 Å

PDB-7yec:
TR-SFX MmCPDII-DNA complex: 6 ns snapshot. Includes 6 ns, dark, and extrapolated structure factors
Method: X-RAY DIFFRACTION / Resolution: 2.2 Å

PDB-7yee:
TR-SFX MmCPDII-DNA complex: 10 ns snapshot. Includes 10 ns, dark, and extrapolated structure factors. Collected at SwissFEL
Method: X-RAY DIFFRACTION / Resolution: 2.15 Å

PDB-7yei:
TR-SFX MmCPDII-DNA complex: 10 ns time-point collected in SACLA. Includes 10 ns, dark, and extrapolated structure factors
Method: X-RAY DIFFRACTION / Resolution: 2.7 Å

PDB-7yej:
TR-SFX MmCPDII-DNA complex: 100 ns time-point collected in SACLA. Includes 100 ns, dark, and extrapolated structure factors
Method: X-RAY DIFFRACTION / Resolution: 2.55 Å

PDB-7yek:
TR-SFX MmCPDII-DNA complex: 500 ns time-point collected in SACLA. Includes 500 ns, dark, and extrapolated structure factors
Method: X-RAY DIFFRACTION / Resolution: 2.4 Å

PDB-7yel:
TR-SFX MmCPDII-DNA complex: 25 us time-point collected in SACLA. Includes 25 us, dark, and extrapolated structure factors
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

PDB-7yem:
TR-SFX MmCPDII-DNA complex: 200 us time-point collected in SACLA. Includes 200 us, dark, and extrapolated structure factors
Method: X-RAY DIFFRACTION / Resolution: 2.6 Å

PDB-8kcm:
MmCPDII-DNA complex containing low-dosage, light induced repaired DNA.
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

Chemicals

ChemComp-FAD:
FLAVIN-ADENINE DINUCLEOTIDE / FAD*YM

ChemComp-SO4:
SULFATE ION

ChemComp-HOH:
WATER

ChemComp-GOL:
GLYCEROL

Source
  • methanosarcina mazei (archaea)
  • synthetic construct (others)
KeywordsDNA BINDING PROTEIN / Flavoprotein / photolyase / light driven electron transfer / DNA repair / time-resolved serial crystallography. / time-resolved serial crystallography

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