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Structure paper

TitleNew mechanistic insight of cytochrome P450 PikC gained from site-specific mutagenesis by non-coding amino acids
Journal, issue, pagesNat Commun, Year 2023
Publish dateMar 21, 2022 (structure data deposition date)
AuthorsPan, Y.J. / Li, G.B. / Gao, X. / Li, S.Y.
External linksSearch PubMed
MethodsX-ray diffraction
Resolution2 - 2.2 Å
Structure data

PDB-7xbn:
Crystal Structure of YC-17-bound cytochrome P450 PikC with the unnatural amino acid p-Acetyl-L-Phenylalanine incorporated at position 238
Method: X-RAY DIFFRACTION / Resolution: 2 Å

PDB-7xbo:
Crystal Structure of 10-dml-bound cytochrome P450 PikC with the unnatural amino acid p-Acetyl-L-Phenylalanine incorporated at position 238
Method: X-RAY DIFFRACTION / Resolution: 2.2 Å

Chemicals

ChemComp-HEM:
PROTOPORPHYRIN IX CONTAINING FE

ChemComp-PXI:
4-{[4-(DIMETHYLAMINO)-3-HYDROXY-6-METHYLTETRAHYDRO-2H-PYRAN-2-YL]OXY}-12-ETHYL-3,5,7,11-TETRAMETHYLOXACYCLODODEC-9-ENE-2,8-DIONE

ChemComp-GOL:
GLYCEROL

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

ChemComp-HOH:
WATER

ChemComp-E4H:
(3R,4S,5S,7R,9E,11R,12R)-12-ETHYL-4-HYDROXY-3,5,7,11-TETRAMETHYLOXACYCLODODEC-9-ENE-2,8-DIONE

Source
  • streptomyces venezuelae (bacteria)
KeywordsOXIDOREDUCTASE / YC-17-bound / PikC / unnatural amino acid / 10-dml-bound

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