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Structure paper

TitleCrystal structures of a 6-dimethylallyltryptophan synthase, IptA: Insights into substrate tolerance and enhancement of prenyltransferase activity.
Journal, issue, pagesBiochem. Biophys. Res. Commun., Vol. 593, Page 144-150, Year 2022
Publish dateDec 8, 2021 (structure data deposition date)
AuthorsSuemune, H. / Nishimura, D. / Mizutani, K. / Sato, Y. / Hino, T. / Takagi, H. / Shiozaki-Sato, Y. / Takahashi, S. / Nagano, S.
External linksBiochem. Biophys. Res. Commun. / PubMed:35074664
MethodsX-ray diffraction
Resolution1.39 - 2.25 Å
Structure data

PDB-7w8u:
Crystal Structure of Indole Prenyltransferase IptA
Method: X-RAY DIFFRACTION / Resolution: 2.25 Å

PDB-7w8v:
DMSPP- and Trp-bound 6-dimethylallyl tryptophan synthase, IptA
Method: X-RAY DIFFRACTION / Resolution: 1.61 Å

PDB-7w8w:
DMSPP- and 5-Me-Trp-bound 6-dimethylallyl tryptophan synthase, IptA
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

PDB-7w8x:
DMSPP- and 6-Me-Trp-bound dimethylallyl tryptophan synthase, IptA
Method: X-RAY DIFFRACTION / Resolution: 1.45 Å

PDB-7w8y:
DMSPP- and Naplha-Me-Trp-bound 6-dimethylallyl tryptophan synthase, IptA
Method: X-RAY DIFFRACTION / Resolution: 1.39 Å

Chemicals

ChemComp-HOH:
WATER

ChemComp-DST:
DIMETHYLALLYL S-THIOLODIPHOSPHATE

ChemComp-SO4:
SULFATE ION

ChemComp-TRP:
TRYPTOPHAN

ChemComp-D0Q:
5-methyl-L-tryptophan

ChemComp-8IK:
(2S)-2-azanyl-3-(6-methyl-1H-indol-3-yl)propanoic acid

ChemComp-TRS:
2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL / pH buffer*YM

ChemComp-DTT:
2,3-DIHYDROXY-1,4-DITHIOBUTANE

ChemComp-E9M:
N-methyl-L-tryptophan

Source
  • streptomyces sp. sn-593 (bacteria)
KeywordsTRANSFERASE / aromatic prenyltransferase / stpreptomyces / secondary metabolites

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