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TitleStructures and mechanisms of actin ATP hydrolysis.
Journal, issue, pagesProc. Natl. Acad. Sci. USA, Vol. 119, Page e2122641119-e2122641119, Year 2022
Publish dateNov 29, 2021 (structure data deposition date)
AuthorsKanematsu, Y. / Narita, A. / Oda, T. / Koike, R. / Ota, M. / Takano, Y. / Moritsugu, K. / Fujiwara, I. / Tanaka, K. / Komatsu, H. ...Kanematsu, Y. / Narita, A. / Oda, T. / Koike, R. / Ota, M. / Takano, Y. / Moritsugu, K. / Fujiwara, I. / Tanaka, K. / Komatsu, H. / Nagae, T. / Watanabe, N. / Iwasa, M. / Maeda, Y. / Takeda, S.
External linksProc. Natl. Acad. Sci. USA / PubMed:36252034
MethodsX-ray diffraction
Resolution1.15 - 2.7 Å
Structure data

PDB-7w4z:
Crystal structure of fragmin domain-1 in complex with actin (AMPPNP-form)
Method: X-RAY DIFFRACTION / Resolution: 1.15 Å

PDB-7w50:
Crystal structure of fragmin domain-1 in complex with actin (ADP-Pi-form)
Method: X-RAY DIFFRACTION / Resolution: 1.15 Å

PDB-7w51:
Crystal structure of fragmin domain-1 in complex with actin (ADP-form)
Method: X-RAY DIFFRACTION / Resolution: 1.2 Å

PDB-7w52:
Crystal structure of fragmin domain-1 (15-160) in complex with actin
Method: X-RAY DIFFRACTION / Resolution: 2.001 Å

PDB-7yne:
Crystal structure of fragmin domain-1 (1-160) in complex with G-form actin
Method: X-RAY DIFFRACTION / Resolution: 2.7 Å

Chemicals

ChemComp-ANP:
PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / AMP-PNP, energy-carrying molecule analogue*YM

ChemComp-MG:
Unknown entry

ChemComp-PO4:
PHOSPHATE ION

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-CA:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM

ChemComp-ACT:
ACETATE ION

ChemComp-NA:
Unknown entry

Source
  • physarum polycephalum (eukaryote)
  • gallus gallus (chicken)
KeywordsCONTRACTILE PROTEIN / actin dynamics / fragmin / gelsolin / ATP hydrolysis

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