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TitleHigh-density binding to Plasmodium falciparum circumsporozoite protein repeats by inhibitory antibody elicited in mouse with human immunoglobulin repertoire.
Journal, issue, pagesPLoS Pathog, Vol. 18, Issue 11, Page e1010999, Year 2022
Publish dateNov 28, 2022
AuthorsIga Kucharska / Špela Binter / Rajagopal Murugan / Stephen W Scally / Julia Ludwig / Katherine Prieto / Elaine Thai / Giulia Costa / Kan Li / Gillian Q Horn / Yevel Flores-Garcia / Alexandre Bosch / Taylor Sicard / John L Rubinstein / Fidel Zavala / S Moses Dennison / Georgia D Tomaras / Elena A Levashina / Paul Kellam / Hedda Wardemann / Jean-Philippe Julien /
PubMed AbstractAntibodies targeting the human malaria parasite Plasmodium falciparum circumsporozoite protein (PfCSP) can prevent infection and disease. PfCSP contains multiple central repeating NANP motifs; some ...Antibodies targeting the human malaria parasite Plasmodium falciparum circumsporozoite protein (PfCSP) can prevent infection and disease. PfCSP contains multiple central repeating NANP motifs; some of the most potent anti-infective antibodies against malaria bind to these repeats. Multiple antibodies can bind the repeating epitopes concurrently by engaging into homotypic Fab-Fab interactions, which results in the ordering of the otherwise largely disordered central repeat into a spiral. Here, we characterize IGHV3-33/IGKV1-5-encoded monoclonal antibody (mAb) 850 elicited by immunization of transgenic mice with human immunoglobulin loci. mAb 850 binds repeating NANP motifs with picomolar affinity, potently inhibits Plasmodium falciparum (Pf) in vitro and, when passively administered in a mouse challenge model, reduces liver burden to a similar extent as some of the most potent anti-PfCSP mAbs yet described. Like other IGHV3-33/IGKV1-5-encoded anti-NANP antibodies, mAb 850 primarily utilizes its HCDR3 and germline-encoded aromatic residues to recognize its core NANP motif. Biophysical and cryo-electron microscopy analyses reveal that up to 19 copies of Fab 850 can bind the PfCSP repeat simultaneously, and extensive homotypic interactions are observed between densely-packed PfCSP-bound Fabs to indirectly improve affinity to the antigen. Together, our study expands on the molecular understanding of repeat-induced homotypic interactions in the B cell response against PfCSP for potently protective mAbs against Pf infection.
External linksPLoS Pathog / PubMed:36441829 / PubMed Central
MethodsEM (single particle) / X-ray diffraction
Resolution2 - 3.4 Å
Structure data

26936

7v05

EMDB-26936, PDB-7v05:
Complex of Plasmodium falciparum circumsporozoite protein with 850 Fab
Method: EM (single particle) / Resolution: 3.4 Å

PDB-7uyl:
850 Fab
Method: X-RAY DIFFRACTION / Resolution: 2.0 Å

PDB-7uym:
850 Fab in complex with NANPNANPNANP peptide
Method: X-RAY DIFFRACTION / Resolution: 2.2 Å

Chemicals

ChemComp-GOL:
GLYCEROL

ChemComp-HOH:
WATER

Source
  • plasmodium falciparum (malaria parasite P. falciparum)
  • mus musculus (house mouse)
  • lama glama (llama)
KeywordsANTIMICROBIAL PROTEIN / IMMUNE SYSTEM / antibody / malaria / Plasmodium falciparum / circumsporozoite protein / IMMUNE SYSTEM/CELL INVASION / IMMUNE SYSTEM-CELL INVASION complex

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