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TitleCryo EM structures map a post vaccination polyclonal antibody response to canine parvovirus.
Journal, issue, pagesCommun Biol, Vol. 6, Issue 1, Page 955, Year 2023
Publish dateSep 19, 2023
AuthorsSamantha R Hartmann / Andrew J Charnesky / Simon P Früh / Robert A López-Astacio / Wendy S Weichert / Nadia DiNunno / Sung Hung Cho / Carol M Bator / Colin R Parrish / Susan L Hafenstein /
PubMed AbstractCanine parvovirus (CPV) is an important pathogen that emerged by cross-species transmission to cause severe disease in dogs. To understand the host immune response to vaccination, sera from dogs ...Canine parvovirus (CPV) is an important pathogen that emerged by cross-species transmission to cause severe disease in dogs. To understand the host immune response to vaccination, sera from dogs immunized with parvovirus are obtained, the polyclonal antibodies are purified and used to solve the high resolution cryo EM structures of the polyclonal Fab-virus complexes. We use a custom software, Icosahedral Subparticle Extraction and Correlated Classification (ISECC) to perform subparticle analysis and reconstruct polyclonal Fab-virus complexes from two different dogs eight and twelve weeks post vaccination. In the resulting polyclonal Fab-virus complexes there are a total of five distinct Fabs identified. In both cases, any of the five antibodies identified would interfere with receptor binding. This polyclonal mapping approach identifies a specific, limited immune response to the live vaccine virus and allows us to investigate the binding of multiple different antibodies or ligands to virus capsids.
External linksCommun Biol / PubMed:37726539 / PubMed Central
MethodsEM (single particle)
Resolution3.0 - 3.8 Å
Structure data

26786

7utp

EMDB-26786, PDB-7utp:
CPV Affinity Purified Polyclonal Fab A Site Fab
Method: EM (single particle) / Resolution: 3.8 Å

26787

7utr

EMDB-26787, PDB-7utr:
CPV Affinity Purified Polyclonal Fab B Site Fab
Method: EM (single particle) / Resolution: 3.7 Å

26788

7uts

EMDB-26788, PDB-7uts:
CPV Total-Fab Polyclonal A Site Fab
Method: EM (single particle) / Resolution: 3.6 Å

26789

7utu

EMDB-26789, PDB-7utu:
CPV Total-Fab Polyclonal B Site Fab (1 of 2)
Method: EM (single particle) / Resolution: 3.0 Å

26790

7utv

EMDB-26790, PDB-7utv:
CPV Total-Fab Polyclonal B Site Fab (2 of 2)
Method: EM (single particle) / Resolution: 3.0 Å

Source
  • canis lupus familiaris (dog)
  • canine parvovirus type 2 (isolate dog/united states/cpv-b/1978)
  • canine parvovirus strain b
KeywordsVIRUS/IMMUNE SYSTEM / CPV / polyclonal Fab / A site / vaccination / VIRUS / VIRUS-IMMUNE SYSTEM complex / B site

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