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Structure paper

TitleStructural mechanisms of assembly, permeation, gating, and pharmacology of native human rod CNG channel.
Journal, issue, pagesNeuron, Vol. 110, Issue 1, Page 86-95.e5, Year 2022
Publish dateJan 5, 2022
AuthorsJing Xue / Yan Han / Weizhong Zeng / Youxing Jiang /
PubMed AbstractMammalian cyclic nucleotide-gated (CNG) channels are nonselective cation channels activated by cGMP or cAMP and play essential roles in the signal transduction of the visual and olfactory sensory ...Mammalian cyclic nucleotide-gated (CNG) channels are nonselective cation channels activated by cGMP or cAMP and play essential roles in the signal transduction of the visual and olfactory sensory systems. CNGA1, the principal component of the CNG channel from rod photoreceptors, can by itself form a functional homotetrameric channel and has been used as the model system in the majority of rod CNG studies. However, the native rod CNG functions as a heterotetramer consisting of three A1 and one B1 subunits and exhibits different functional properties than the CNGA1 homomer. Here we present the functional analysis of human rod CNGA1/B1 heterotetramer and its cryo-EM structures in apo, cGMP-bound, cAMP-bound, and L-cis-Diltiazem-blocked states. These structures, with resolution ranging from 2.6 to 3.3 Å, elucidate the structural mechanisms underlying the 3:1 subunit stoichiometry, the asymmetrical gating upon cGMP activation, and the unique pharmacological property of the native rod CNG channel.
External linksNeuron / PubMed:34699778 / PubMed Central
MethodsEM (single particle)
Resolution2.61 - 3.31 Å
Structure data

24458

7rh9

EMDB-24458, PDB-7rh9:
Cryo-EM structure of human rod CNGA1/B1 channel in apo state
Method: EM (single particle) / Resolution: 2.61 Å

24460

7rhg

EMDB-24460, PDB-7rhg:
Cryo-EM structure of human rod CNGA1/B1 channel in cAMP-bound state
Method: EM (single particle) / Resolution: 2.88 Å

24461

7rhh

EMDB-24461, PDB-7rhh:
Cryo-EM structure of human rod CNGA1/B1 channel in cGMP-bound openI state
Method: EM (single particle) / Resolution: 3.31 Å

24462

7rhi

EMDB-24462, PDB-7rhi:
Cryo-EM structure of human rod CNGA1/B1 channel in cGMP-bound openII state
Method: EM (single particle) / Resolution: 3.31 Å

24463

7rhj

EMDB-24463, PDB-7rhj:
Cryo-EM structure of human rod CNGA1/B1 channel in L-cis-Diltiazem-blocked open state
Method: EM (single particle) / Resolution: 2.88 Å

24464

7rhk

EMDB-24464, PDB-7rhk:
Cryo-EM structure of human rod CNGA1/B1 channel in L-cis-Diltiazem-trapped closed state
Method: EM (single particle) / Resolution: 3.27 Å

24465

7rhl

EMDB-24465, PDB-7rhl:
Cryo-EM structure of human rod Apo CNGA1/B1 channel with CLZ coiled coil
Method: EM (single particle) / Resolution: 3.03 Å

Chemicals

ChemComp-CMP:
ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE

ChemComp-PCG:
CYCLIC GUANOSINE MONOPHOSPHATE

ChemComp-5H0:
(2R,3R)-5-[2-(dimethylamino)ethyl]-2-(4-methoxyphenyl)-4-oxo-2,3,4,5-tetrahydro-1,5-benzothiazepin-3-yl acetate

Source
  • homo sapiens (human)
KeywordsTRANSPORT PROTEIN / ion channel

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