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TitleA hold-and-feed mechanism drives directional DNA loop extrusion by condensin.
Journal, issue, pagesScience, Vol. 376, Issue 6597, Page 1087-1094, Year 2022
Publish dateJun 3, 2022
AuthorsIndra A Shaltiel / Sumanjit Datta / Léa Lecomte / Markus Hassler / Marc Kschonsak / Sol Bravo / Catherine Stober / Jenny Ormanns / Sebastian Eustermann / Christian H Haering /
PubMed AbstractStructural maintenance of chromosomes (SMC) protein complexes structure genomes by extruding DNA loops, but the molecular mechanism that underlies their activity has remained unknown. We show that ...Structural maintenance of chromosomes (SMC) protein complexes structure genomes by extruding DNA loops, but the molecular mechanism that underlies their activity has remained unknown. We show that the active condensin complex entraps the bases of a DNA loop transiently in two separate chambers. Single-molecule imaging and cryo-electron microscopy suggest a putative power-stroke movement at the first chamber that feeds DNA into the SMC-kleisin ring upon adenosine triphosphate binding, whereas the second chamber holds on upstream of the same DNA double helix. Unlocking the strict separation of "motor" and "anchor" chambers turns condensin from a one-sided into a bidirectional DNA loop extruder. We conclude that the orientation of two topologically bound DNA segments during the SMC reaction cycle determines the directionality of DNA loop extrusion.
External linksScience / PubMed:35653469
MethodsEM (single particle)
Resolution3.46 - 3.86 Å
Structure data

13934

7qen

EMDB-13934, PDB-7qen:
S.c. Condensin core in DNA- and ATP-bound state
Method: EM (single particle) / Resolution: 3.46 Å

13950

7qfw

EMDB-13950, PDB-7qfw:
S.c. Condensin peripheral Ycg1 subcomplex bound to DNA
Method: EM (single particle) / Resolution: 3.86 Å

Chemicals

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM

ChemComp-MG:
Unknown entry

Source
  • saccharomyces cerevisiae (brewer's yeast)
  • synthetic construct (others)
  • saccharomyces cerevisiae cen.pk113-7d (yeast)
  • saccharomyces cerevisiae s288c (yeast)
KeywordsDNA BINDING PROTEIN / SMC-motor protein

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