Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	High-resolution structures of the actomyosin-V complex in three nucleotide states provide insights into the force generation mechanism.
Journal, issue, pages	Elife, Vol. 10, Year 2021
Publish date	Nov 23, 2021
Authors	Sabrina Pospich / H Lee Sweeney / Anne Houdusse / Stefan Raunser /
PubMed Abstract	The molecular motor myosin undergoes a series of major structural transitions during its force-producing motor cycle. The underlying mechanism and its coupling to ATP hydrolysis and actin binding are ...The molecular motor myosin undergoes a series of major structural transitions during its force-producing motor cycle. The underlying mechanism and its coupling to ATP hydrolysis and actin binding are only partially understood, mostly due to sparse structural data on actin-bound states of myosin. Here, we report 26 high-resolution cryo-EM structures of the actomyosin-V complex in the strong-ADP, rigor, and a previously unseen post-rigor transition state that binds the ATP analog AppNHp. The structures reveal a high flexibility of myosin in each state and provide valuable insights into the structural transitions of myosin-V upon ADP release and binding of AppNHp, as well as the actomyosin interface. In addition, they show how myosin is able to specifically alter the structure of F-actin.
External links	Elife / PubMed:34812732 / PubMed Central
Methods	EM (helical sym.)
Resolution	2.9 - 3.7 Å
Structure data	13501 7plt EMDB-13501, PDB-7plt: Cryo-EM structure of the actomyosin-V complex in the rigor state (central 1er) Method: EM (helical sym.) / Resolution: 3.3 Å 13502 7plu EMDB-13502, PDB-7plu: Cryo-EM structure of the actomyosin-V complex in the rigor state (central 3er/2er) Method: EM (helical sym.) / Resolution: 3.2 Å 13503 7plv EMDB-13503, PDB-7plv: Cryo-EM structure of the actomyosin-V complex in the rigor state (central 1er, class 1) Method: EM (helical sym.) / Resolution: 3.5 Å 13504 7plw EMDB-13504, PDB-7plw: Cryo-EM structure of the actomyosin-V complex in the rigor state (central 1er, class 2) Method: EM (helical sym.) / Resolution: 3.5 Å 13505 7plx EMDB-13505, PDB-7plx: Cryo-EM structure of the actomyosin-V complex in the rigor state (central 1er, class 4) Method: EM (helical sym.) / Resolution: 3.6 Å 13506 7ply EMDB-13506, PDB-7ply: Cryo-EM structure of the actomyosin-V complex in the rigor state (central 1er, young JASP-stabilized F-actin) Method: EM (helical sym.) / Resolution: 3.2 Å 13507 7plz EMDB-13507, PDB-7plz: Cryo-EM structure of the actomyosin-V complex in the rigor state (central 3er/2er, young JASP-stabilized F-actin) Method: EM (helical sym.) / Resolution: 3.2 Å 13508 7pm0 EMDB-13508, PDB-7pm0: Cryo-EM structure of the actomyosin-V complex in the rigor state (central 1er, young JASP-stabilized F-actin, class 1) Method: EM (helical sym.) / Resolution: 3.6 Å 13509 7pm1 EMDB-13509, PDB-7pm1: Cryo-EM structure of the actomyosin-V complex in the rigor state (central 1er, young JASP-stabilized F-actin, class 2) Method: EM (helical sym.) / Resolution: 3.5 Å 13510 7pm2 EMDB-13510, PDB-7pm2: Cryo-EM structure of the actomyosin-V complex in the rigor state (central 1er, young JASP-stabilized F-actin, class 4) Method: EM (helical sym.) / Resolution: 3.6 Å 13511 7pm3 EMDB-13511, PDB-7pm3: Cryo-EM structure of young JASP-stabilized F-actin (central 3er) Method: EM (helical sym.) / Resolution: 3.1 Å 13521 7pm5 EMDB-13521, PDB-7pm5: Cryo-EM structure of the actomyosin-V complex in the strong-ADP state (central 1er) Method: EM (helical sym.) / Resolution: 3.1 Å 13522 7pm6 EMDB-13522, PDB-7pm6: Cryo-EM structure of the actomyosin-V complex in the strong-ADP state (central 3er/2er) Method: EM (helical sym.) / Resolution: 3.0 Å 13523 7pm7 EMDB-13523, PDB-7pm7: Cryo-EM structure of the actomyosin-V complex in the strong-ADP state (central 1er, class 2) Method: EM (helical sym.) / Resolution: 3.5 Å 13524 7pm8 EMDB-13524, PDB-7pm8: Cryo-EM structure of the actomyosin-V complex in the strong-ADP state (central 1er, class 3) Method: EM (helical sym.) / Resolution: 3.5 Å 13525 7pm9 EMDB-13525, PDB-7pm9: Cryo-EM structure of the actomyosin-V complex in the strong-ADP state (central 1er, class 4) Method: EM (helical sym.) / Resolution: 3.7 Å 13526 7pma EMDB-13526, PDB-7pma: Cryo-EM structure of the actomyosin-V complex in the strong-ADP state (central 1er, class 5) Method: EM (helical sym.) / Resolution: 3.6 Å 13527 7pmb EMDB-13527, PDB-7pmb: Cryo-EM structure of the actomyosin-V complex in the strong-ADP state (central 1er, class 6) Method: EM (helical sym.) / Resolution: 3.6 Å 13528 7pmc EMDB-13528, PDB-7pmc: Cryo-EM structure of the actomyosin-V complex in the strong-ADP state (central 1er, class 7) Method: EM (helical sym.) / Resolution: 3.7 Å 13529 7pmd EMDB-13529, PDB-7pmd: Cryo-EM structure of the actomyosin-V complex in the post-rigor transition state (AppNHp, central 1er) Method: EM (helical sym.) / Resolution: 2.9 Å 13530 7pme EMDB-13530, PDB-7pme: Cryo-EM structure of the actomyosin-V complex in the post-rigor transition state (AppNHp, central 3er/2er) Method: EM (helical sym.) / Resolution: 2.9 Å 13531 7pmf EMDB-13531, PDB-7pmf: Cryo-EM structure of the actomyosin-V complex in the post-rigor transition state (AppNHp, central 1er, class 1) Method: EM (helical sym.) / Resolution: 3.4 Å 13532 7pmg EMDB-13532, PDB-7pmg: Cryo-EM structure of the actomyosin-V complex in the post-rigor transition state (AppNHp, central 1er, class 3) Method: EM (helical sym.) / Resolution: 3.3 Å 13533 7pmh EMDB-13533, PDB-7pmh: Cryo-EM structure of the actomyosin-V complex in the post-rigor transition state (AppNHp, central 1er, class 4) Method: EM (helical sym.) / Resolution: 3.4 Å 13535 7pmi EMDB-13535, PDB-7pmi: Cryo-EM structure of the actomyosin-V complex in the post-rigor transition state (AppNHp, central 1er, class 5) Method: EM (helical sym.) / Resolution: 3.3 Å 13536 7pmj EMDB-13536, PDB-7pmj: Cryo-EM structure of the actomyosin-V complex in the post-rigor transition state (AppNHp, central 1er, class 6) Method: EM (helical sym.) / Resolution: 3.4 Å 13538 7pml EMDB-13538, PDB-7pml: Cryo-EM structure of the actomyosin-V complex in the post-rigor transition state (AppNHp, central 1er, class 8) Method: EM (helical sym.) / Resolution: 3.3 Å
Chemicals	ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying moleculeYM ChemComp-MG: Unknown entry ChemComp-PO4: PHOSPHATE ION ChemComp-9UE: Jasplakinolide ChemComp-ANP: PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / AMP-PNP, energy-carrying molecule analogueYM
Source	gallus gallus (chicken) Rabbit (rabbit) amanita phalloides (death cap) homo sapiens (human) oryctolagus cuniculus (rabbit) synthetic construct (others)
Keywords	MOTOR PROTEIN / myosin / cytoskeleton / F-actin / phalloidin / jasplakinolide / STRUCTURAL PROTEIN