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Structure paper

TitleStructural basis for PoxtA-mediated resistance to phenicol and oxazolidinone antibiotics.
Journal, issue, pagesNat Commun, Vol. 13, Issue 1, Page 1860, Year 2022
Publish dateApr 6, 2022
AuthorsCaillan Crowe-McAuliffe / Victoriia Murina / Kathryn Jane Turnbull / Susanne Huch / Marje Kasari / Hiraku Takada / Lilit Nersisyan / Arnfinn Sundsfjord / Kristin Hegstad / Gemma C Atkinson / Vicent Pelechano / Daniel N Wilson / Vasili Hauryliuk /
PubMed AbstractPoxtA and OptrA are ATP binding cassette (ABC) proteins of the F subtype (ABCF). They confer resistance to oxazolidinone and phenicol antibiotics, such as linezolid and chloramphenicol, which stall ...PoxtA and OptrA are ATP binding cassette (ABC) proteins of the F subtype (ABCF). They confer resistance to oxazolidinone and phenicol antibiotics, such as linezolid and chloramphenicol, which stall translating ribosomes when certain amino acids are present at a defined position in the nascent polypeptide chain. These proteins are often encoded on mobile genetic elements, facilitating their rapid spread amongst Gram-positive bacteria, and are thought to confer resistance by binding to the ribosome and dislodging the bound antibiotic. However, the mechanistic basis of this resistance remains unclear. Here we refine the PoxtA spectrum of action, demonstrate alleviation of linezolid-induced context-dependent translational stalling, and present cryo-electron microscopy structures of PoxtA in complex with the Enterococcus faecalis 70S ribosome. PoxtA perturbs the CCA-end of the P-site tRNA, causing it to shift by ∼4 Å out of the ribosome, corresponding to a register shift of approximately one amino acid for an attached nascent polypeptide chain. We postulate that the perturbation of the P-site tRNA by PoxtA thereby alters the conformation of the attached nascent chain to disrupt the drug binding site.
External linksNat Commun / PubMed:35387982 / PubMed Central
MethodsEM (single particle)
Resolution2.4 - 3.1 Å
Structure data

13241

7p7q

EMDB-13241, PDB-7p7q:
E. faecalis 70S ribosome bound by PoxtA-EQ2, high-resolution combined volume
Method: EM (single particle) / Resolution: 2.4 Å

13242

7p7r

EMDB-13242, PDB-7p7r:
PoxtA-EQ2 antibiotic resistance ABCF bound to E. faecalis 70S ribosome, state I
Method: EM (single particle) / Resolution: 2.9 Å

13243

7p7s

EMDB-13243, PDB-7p7s:
PoxtA-EQ2 antibiotic resistance ABCF bound to E. faecalis 70S ribosome, state II
Method: EM (single particle) / Resolution: 3.0 Å

13244

7p7t

EMDB-13244, PDB-7p7t:
PoxtA-EQ2 antibiotic resistance ABCF bound to E. faecalis 70S ribosome, state III
Method: EM (single particle) / Resolution: 2.9 Å

13245

7p7u

EMDB-13245, PDB-7p7u:
E. faecalis 70S ribosome with P-tRNA, state IV
Method: EM (single particle) / Resolution: 3.1 Å

Chemicals

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM

ChemComp-MG:
Unknown entry

ChemComp-K:
Unknown entry

ChemComp-ZN:
Unknown entry

ChemComp-PUT:
1,4-DIAMINOBUTANE

ChemComp-SCM:
SPECTINOMYCIN / antibiotic*YM

ChemComp-HOH:
WATER

Source
  • enterococcus faecalis (bacteria)
  • enterococcus faecalis v583 (bacteria)
  • enterococcus faecalis (strain atcc 700802 / v583) (bacteria)
  • escherichia coli (E. coli)
KeywordsRIBOSOME / Enterococcus faecalis / PoxtA / ABCF / antibiotic resistance protein / P-tRNA

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