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TitleMicroenvironment-Sensitive Fluorescent Nucleotide Probes from Benzofuran, Benzothiophene, and Selenophene as Substrates for DNA Polymerases.
Journal, issue, pagesJ. Am. Chem. Soc., Vol. 144, Page 10556-10569, Year 2022
Publish dateJun 18, 2021 (structure data deposition date)
AuthorsGhosh, P. / Kropp, H.M. / Betz, K. / Ludmann, S. / Diederichs, K. / Marx, A. / Srivatsan, S.G.
External linksJ. Am. Chem. Soc. / PubMed:35666775
MethodsX-ray diffraction
Resolution1.91 Å
Structure data

PDB-7owf:
KlenTaq DNA polymerase in a ternary complex with primer/template and the fluorescent nucleotide analog BFdUTP
Method: X-RAY DIFFRACTION / Resolution: 1.91 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-2IU:
[[(2R,3S,5R)-5-[5-(1-benzofuran-2-yl)-2,4-bis(oxidanylidene)pyrimidin-1-yl]-3-oxidanyl-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] phosphono hydrogen phosphate

ChemComp-GOL:
GLYCEROL

ChemComp-HOH:
WATER

Source
  • thermus aquaticus (bacteria)
  • synthetic construct (others)
KeywordsTRANSFERASE / DNA Polymerase / KlenTaq / KTQ / artificial substrate

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