Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	A distinct assembly pathway of the human 39S late pre-mitoribosome.
Journal, issue, pages	Nat Commun, Vol. 12, Issue 1, Page 4544, Year 2021
Publish date	Jul 27, 2021
Authors	Jingdong Cheng / Otto Berninghausen / Roland Beckmann /
PubMed Abstract	Assembly of the mitoribosome is largely enigmatic and involves numerous assembly factors. Little is known about their function and the architectural transitions of the pre-ribosomal intermediates. ...Assembly of the mitoribosome is largely enigmatic and involves numerous assembly factors. Little is known about their function and the architectural transitions of the pre-ribosomal intermediates. Here, we solve cryo-EM structures of the human 39S large subunit pre-ribosomes, representing five distinct late states. Besides the MALSU1 complex used as bait for affinity purification, we identify several assembly factors, including the DDX28 helicase, MRM3, GTPBP10 and the NSUN4-mTERF4 complex, all of which keep the 16S rRNA in immature conformations. The late transitions mainly involve rRNA domains IV and V, which form the central protuberance, the intersubunit side and the peptidyltransferase center of the 39S subunit. Unexpectedly, we find deacylated tRNA in the ribosomal E-site, suggesting a role in 39S assembly. Taken together, our study provides an architectural inventory of the distinct late assembly phase of the human 39S mitoribosome.
External links	Nat Commun / PubMed:34315873 / PubMed Central
Methods	EM (single particle)
Resolution	3.1 - 5.7 Å
Structure data	12919 7oi6 EMDB-12919, PDB-7oi6: Cryo-EM structure of late human 39S mitoribosome assembly intermediates, state 1 Method: EM (single particle) / Resolution: 5.7 Å 12920 7oi7 EMDB-12920, PDB-7oi7: Cryo-EM structure of late human 39S mitoribosome assembly intermediates, state 2 Method: EM (single particle) / Resolution: 3.5 Å 12921 7oi8 EMDB-12921, PDB-7oi8: Cryo-EM structure of late human 39S mitoribosome assembly intermediates, state 3A Method: EM (single particle) / Resolution: 3.5 Å 12922 7oi9 EMDB-12922, PDB-7oi9: Cryo-EM structure of late human 39S mitoribosome assembly intermediates, state 3B Method: EM (single particle) / Resolution: 3.3 Å 12923 7oia EMDB-12923, PDB-7oia: Cryo-EM structure of late human 39S mitoribosome assembly intermediates, state 3C Method: EM (single particle) / Resolution: 3.2 Å 12924 7oib EMDB-12924, PDB-7oib: Cryo-EM structure of late human 39S mitoribosome assembly intermediates, state 3D Method: EM (single particle) / Resolution: 3.3 Å 12925 7oic EMDB-12925, PDB-7oic: Cryo-EM structure of late human 39S mitoribosome assembly intermediates, state 4 Method: EM (single particle) / Resolution: 3.1 Å 12926 7oid EMDB-12926, PDB-7oid: Cryo-EM structure of late human 39S mitoribosome assembly intermediates, state 5A Method: EM (single particle) / Resolution: 3.7 Å 12927 7oie EMDB-12927, PDB-7oie: Cryo-EM structure of late human 39S mitoribosome assembly intermediates, state 5B Method: EM (single particle) / Resolution: 3.5 Å
Chemicals	ChemComp-MG: Unknown entry ChemComp-ZN: Unknown entry ChemComp-PNS: 4'-PHOSPHOPANTETHEINE / Phosphopantetheine ChemComp-C: CYTIDINE-5'-MONOPHOSPHATE / Cytidine monophosphate ChemComp-A: ADENOSINE-5'-MONOPHOSPHATE / Adenosine monophosphate ChemComp-SAM: S-ADENOSYLMETHIONINE / S-Adenosyl methionine
Source	homo sapiens (human) Human (human)
Keywords	RIBOSOME / 39S mitoribosome / ribosome biogenesis