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TitleStructural Basis for a Neutralizing Antibody Response Elicited by a Recombinant Hantaan Virus Gn Immunogen.
Journal, issue, pagesmBio, Vol. 12, Issue 4, Page e0253120, Year 2021
Publish dateAug 31, 2021
AuthorsIlona Rissanen / Stefanie A Krumm / Robert Stass / Annalis Whitaker / James E Voss / Emily A Bruce / Sylvia Rothenberger / Stefan Kunz / Dennis R Burton / Juha T Huiskonen / Jason W Botten / Thomas A Bowden / Katie J Doores /
PubMed AbstractHantaviruses are a group of emerging pathogens capable of causing severe disease upon zoonotic transmission to humans. The mature hantavirus surface presents higher-order tetrameric assemblies of two ...Hantaviruses are a group of emerging pathogens capable of causing severe disease upon zoonotic transmission to humans. The mature hantavirus surface presents higher-order tetrameric assemblies of two glycoproteins, Gn and Gc, which are responsible for negotiating host cell entry and constitute key therapeutic targets. Here, we demonstrate that recombinantly derived Gn from Hantaan virus (HTNV) elicits a neutralizing antibody response (serum dilution that inhibits 50% infection [ID], 1:200 to 1:850) in an animal model. Using antigen-specific B cell sorting, we isolated monoclonal antibodies (mAbs) exhibiting neutralizing and non-neutralizing activity, termed mAb HTN-Gn1 and mAb nnHTN-Gn2, respectively. Crystallographic analysis reveals that these mAbs target spatially distinct epitopes at disparate sites of the N-terminal region of the HTNV Gn ectodomain. Epitope mapping onto a model of the higher order (Gn-Gc) spike supports the immune accessibility of the mAb HTN-Gn1 epitope, a hypothesis confirmed by electron cryo-tomography of the antibody with virus-like particles. These data define natively exposed regions of the hantaviral Gn that can be targeted in immunogen design. The spillover of pathogenic hantaviruses from rodent reservoirs into the human population poses a continued threat to human health. Here, we show that a recombinant form of the Hantaan virus (HTNV) surface-displayed glycoprotein, Gn, elicits a neutralizing antibody response in rabbits. We isolated a neutralizing (HTN-Gn1) and a non-neutralizing (nnHTN-Gn2) monoclonal antibody and provide the first molecular-level insights into how the Gn glycoprotein may be targeted by the antibody-mediated immune response. These findings may guide rational vaccine design approaches focused on targeting the hantavirus glycoprotein envelope.
External linksmBio / PubMed:34225492 / PubMed Central
MethodsEM (subtomogram averaging) / X-ray diffraction
Resolution2.7 - 19.0 Å
Structure data

EMDB-12543:
Hantaan virus-like particle glycoprotein lattice.
Method: EM (subtomogram averaging) / Resolution: 12.3 Å

12544

7nrh

EMDB-12544: Hantaan virus-like particle glycoprotein spike in complex with Fab fragment HTN-Gn1.
PDB-7nrh: Hantaan virus glycoprotein (Gn) in complex with Fab fragment HTN-Gn1.
Method: EM (subtomogram averaging) / Resolution: 19.0 Å

PDB-7nks:
Structure of the Hantaan virus Gn glycoprotein ectodomain in complex with Fab HTN-Gn1
Method: X-RAY DIFFRACTION / Resolution: 3.5 Å

PDB-7o9s:
Hantaan virus Gn in complex with Fab nnHTN-Gn2
Method: X-RAY DIFFRACTION / Resolution: 2.7 Å

Chemicals

ChemComp-GOL:
GLYCEROL

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose

Source
  • oryctolagus cuniculus (rabbit)
  • hantaan orthohantavirus
KeywordsVIRAL PROTEIN / Hantaan virus glycoprotein / Gn glycoprotein / Fab / neutralizing antibody / viral glycoprotein in complex with antibody / VIRUS LIKE PARTICLE / VLP / virus-like particle / Hantaan / HTNV / HNTV / glycoprotein / spike / HTN-Gn1 / antibody / epitope / Gn in complex with Fab

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