EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Potent SARS-CoV-2 neutralizing antibodies directed against spike N-terminal domain target a single supersite.
ジャーナル・号・ページ	Cell Host Microbe, Vol. 29, Issue 5, Page 819-833.e7, Year 2021
掲載日	2021年5月12日
著者	Gabriele Cerutti / Yicheng Guo / Tongqing Zhou / Jason Gorman / Myungjin Lee / Micah Rapp / Eswar R Reddem / Jian Yu / Fabiana Bahna / Jude Bimela / Yaoxing Huang / Phinikoula S Katsamba / Lihong Liu / Manoj S Nair / Reda Rawi / Adam S Olia / Pengfei Wang / Baoshan Zhang / Gwo-Yu Chuang / David D Ho / Zizhang Sheng / Peter D Kwong / Lawrence Shapiro /
PubMed 要旨	Numerous antibodies that neutralize SARS-CoV-2 have been identified, and these generally target either the receptor-binding domain (RBD) or the N-terminal domain (NTD) of the viral spike. While RBD- ...Numerous antibodies that neutralize SARS-CoV-2 have been identified, and these generally target either the receptor-binding domain (RBD) or the N-terminal domain (NTD) of the viral spike. While RBD-directed antibodies have been extensively studied, far less is known about NTD-directed antibodies. Here, we report cryo-EM and crystal structures for seven potent NTD-directed neutralizing antibodies in complex with spike or isolated NTD. These structures defined several antibody classes, with at least one observed in multiple convalescent donors. The structures revealed that all seven antibodies target a common surface, bordered by glycans N17, N74, N122, and N149. This site-formed primarily by a mobile β-hairpin and several flexible loops-was highly electropositive, located at the periphery of the spike, and the largest glycan-free surface of NTD facing away from the viral membrane. Thus, in contrast to neutralizing RBD-directed antibodies that recognize multiple non-overlapping epitopes, potent NTD-directed neutralizing antibodies appear to target a single supersite.
リンク	Cell Host Microbe / PubMed:33789084 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	2.97 - 4.47 Å
構造データ	23125 7l2d EMDB-23125, PDB-7l2d: Cryo-EM structure of NTD-directed neutralizing antibody 1-87 in complex with prefusion SARS-CoV-2 spike glycoprotein 手法: EM (単粒子) / 解像度: 3.55 Å 23126 7l2e EMDB-23126, PDB-7l2e: Cryo-EM structure of NTD-directed neutralizing antibody 4-18 in complex with prefusion SARS-CoV-2 spike glycoprotein 手法: EM (単粒子) / 解像度: 2.97 Å 23127 7l2f EMDB-23127, PDB-7l2f: Cryo-EM structure of NTD-directed neutralizing antibody 5-24 in complex with prefusion SARS-CoV-2 spike glycoprotein 手法: EM (単粒子) / 解像度: 3.9 Å EMDB-23150: Cryo-EM map of NTD-directed neutralizing antibody 1-68 in complex with prefusion SARS-CoV-2 spike glycoprotein 手法: EM (単粒子) / 解像度: 3.8 Å EMDB-23151: Cryo-EM map of NTD-directed neutralizing antibody 2-51 in complex with prefusion SARS-CoV-2 spike glycoprotein 手法: EM (単粒子) / 解像度: 3.71 Å 23489 7lqv EMDB-23489, PDB-7lqv: Cryo-EM structure of NTD-directed neutralizing antibody 4-8 Fab in complex with SARS-CoV-2 S2P spike 手法: EM (単粒子) / 解像度: 3.25 Å 23490 7lqw EMDB-23490, PDB-7lqw: Cryo-EM structure of NTD-directed neutralizing antibody 2-17 Fab in complex with SARS-CoV-2 S2P spike 手法: EM (単粒子) / 解像度: 4.47 Å PDB-7l2c: Crystallographic structure of neutralizing antibody 2-51 in complex with SARS-CoV-2 spike N-terminal domain (NTD) 手法: X-RAY DIFFRACTION / 解像度: 3.65 Å
化合物	ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン / N-アセチルグルコサミン ChemComp-CA: Unknown entry / カルシウムジカチオン ChemComp-ACT: ACETATE ION / 酢酸イオン / 酢酸塩 ChemComp-CAC: CACODYLATE ION / ジメチルアルシン酸アニオン / カコジル酸 ChemComp-PGE: TRIETHYLENE GLYCOL / トリエチレングリコ-ル / ポリエチレングリコール ChemComp-HOH: WATER / 水
由来	homo sapiens (ヒト) severe acute respiratory syndrome coronavirus 2 (SARSコロナウイルス2)
キーワード	IMMUNE SYSTEM/Viral Protein (免疫系) / COVID-19 (新型コロナウイルス感染症 (2019年)) / SARS-CoV-2 (SARSコロナウイルス2) / Viral protein (ウイルスタンパク質) / Spike glycoprotein (スパイクタンパク質) / N-terminal domain (N末端) / NTD / Neutralizing antibody (中和抗体) / 2-51 / Fab / IMMUNE SYSTEM (免疫系) / IMMUNE SYSTEM-Viral Protein complex (免疫系) / Viral protein/Immune system (ウイルス性) / Fusion protein (融合タンパク質) / NTD-directed antibody / 1-87 / Viral protein-Immune system complex (ウイルス性) / 4-18 / 5-24 / COVID (新型コロナウイルス感染症 (2019年)) / COVID19 (新型コロナウイルス感染症 (2019年)) / SARS-CoV2 (SARSコロナウイルス2) / prefusion / neutralizing (中和抗体)