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Structure paper

TitleStructural and thermodynamic insights into a novel Mg 2+ -citrate-binding protein from the ABC transporter superfamily.
Journal, issue, pagesActa Crystallogr D Struct Biol, Vol. 77, Page 1516-1534, Year 2021
Publish dateJun 25, 2021 (structure data deposition date)
AuthorsMandal, S.K. / Kanaujia, S.P.
External linksActa Crystallogr D Struct Biol / PubMed:34866608
MethodsX-ray diffraction
Resolution1.63 - 2.5 Å
Structure data

PDB-7f6e:
Crystal structure of metal-citrate-binding protein (MctA) of ABC transporter endogenously bound to Mg2+-citrate complex (Form I)
Method: X-RAY DIFFRACTION / Resolution: 1.77 Å

PDB-7f6f:
Crystal structure of metal-citrate-binding protein (MctA) of ABC transporter endogenously bound to Mg2+-citrate complex (Form II)
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

PDB-7f6k:
Crystal structure of metal-citrate-binding protein (MctA) of ABC transporter endogenously bound to citrate
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

PDB-7f6n:
Crystal structure of metal-citrate-binding mutant (S26A) protein (MctA) of ABC transporter endogenously bound to Mg2+-citrate complex
Method: X-RAY DIFFRACTION / Resolution: 2.3 Å

PDB-7f6o:
Crystal structure of metal-citrate-binding mutant (S26A) protein (MctA) of ABC transporter endogenously bound to Mn2+-citrate complex
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

PDB-7f6p:
Crystal structure of metal-citrate-binding mutant (D28A) protein (MctA) of ABC transporter endogenously bound to citrate
Method: X-RAY DIFFRACTION / Resolution: 2.15 Å

PDB-7f6q:
Crystal structure of metal-citrate-binding mutant (S79A) protein (MctA) of ABC transporter in apo state
Method: X-RAY DIFFRACTION / Resolution: 1.63 Å

PDB-7f6r:
Crystal structure of metal-citrate-binding mutant (S164A) protein (MctA) of ABC transporter in apo state
Method: X-RAY DIFFRACTION / Resolution: 2.09 Å

PDB-7f6s:
Crystal structure of metal-citrate-binding mutant (T199A) protein (MctA) of ABC transporter in apo state
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

PDB-7f6t:
Crystal structure of metal-citrate-binding mutant (Y221F) protein (MctA) of ABC transporter in apo state
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-7f6u:
Crystal structure of metal-citrate-binding mutant (Y221A) protein (MctA) of ABC transporter in apo state
Method: X-RAY DIFFRACTION / Resolution: 1.75 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-CIT:
CITRIC ACID

ChemComp-CL:
Unknown entry

ChemComp-CO2:
CARBON DIOXIDE

ChemComp-GOL:
GLYCEROL

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-2OP:
(2S)-2-HYDROXYPROPANOIC ACID

ChemComp-PGO:
S-1,2-PROPANEDIOL

ChemComp-HOH:
WATER

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

ChemComp-MES:
2-(N-MORPHOLINO)-ETHANESULFONIC ACID / pH buffer*YM

ChemComp-ACT:
ACETATE ION

ChemComp-SO4:
SULFATE ION

ChemComp-PGR:
R-1,2-PROPANEDIOL

ChemComp-MN:
Unknown entry

ChemComp-SO3:
SULFITE ION

ChemComp-PG4:
TETRAETHYLENE GLYCOL / precipitant*YM

ChemComp-SO2:
SULFUR DIOXIDE

ChemComp-PDO:
1,3-PROPANDIOL

ChemComp-CO3:
CARBONATE ION

ChemComp-NA:
Unknown entry

ChemComp-GOA:
GLYCOLIC ACID

ChemComp-PO4:
PHOSPHATE ION

ChemComp-BGQ:
2-HYDROXY BUTANE-1,4-DIOL

ChemComp-1MI:
2-(hydroxymethyl)butane-1,4-diol

ChemComp-CAC:
CACODYLATE ION

Source
  • thermus thermophilus (strain atcc 27634 / dsm 579 / hb8) (bacteria)
KeywordsTRANSPORT PROTEIN / Substrate-binding protein / metal ion / secondary transporter / symporter

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