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TitleMechanistic analysis of carbon-carbon bond formation by deoxypodophyllotoxin synthase.
Journal, issue, pagesProc. Natl. Acad. Sci. USA, Vol. 119, Year 2022
Publish dateFeb 8, 2021 (structure data deposition date)
AuthorsTang, H. / Wu, M.H. / Lin, H.Y. / Han, M.R. / Tu, Y.H. / Yang, Z.J. / Chien, T.C. / Chan, N.L. / Chang, W.C.
External linksProc. Natl. Acad. Sci. USA / PubMed:34969844
MethodsX-ray diffraction
Resolution2.05 - 2.09 Å
Structure data

PDB-7e37:
Crystal structure of deoxypodophyllotoxin synthase from Sinopodophyllum hexandrum in complex with 2-oxoglutarate
Method: X-RAY DIFFRACTION / Resolution: 2.09 Å

PDB-7e38:
Crystal structure of deoxypodophyllotoxin synthase from Sinopodophyllum hexandrum in complex with yatein and succinate
Method: X-RAY DIFFRACTION / Resolution: 2.05 Å

Chemicals

ChemComp-FE:
Unknown entry

ChemComp-AKG:
2-OXOGLUTARIC ACID

ChemComp-HOH:
WATER

ChemComp-SIN:
SUCCINIC ACID

ChemComp-YTC:
(3~{S},4~{S})-4-(1,3-benzodioxol-5-ylmethyl)-3-[(3,4,5-trimethoxyphenyl)methyl]oxolan-2-one

ChemComp-YTN:
(3~{R},4~{R})-4-(1,3-benzodioxol-5-ylmethyl)-3-[(3,4,5-trimethoxyphenyl)methyl]oxolan-2-one

Source
  • sinopodophyllum hexandrum (Himalayan mayapple)
KeywordsBIOSYNTHETIC PROTEIN / non-heme iron/2-oxoglutarate enzyme beta-helix fold deoxypodophyllotoxin biosynthesis carbon-carbon bond formation

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