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Structure paper

TitleStructural basis of trehalose recycling by the ABC transporter LpqY-SugABC.
Journal, issue, pagesSci Adv, Vol. 6, Issue 44, Year 2020
Publish dateOct 30, 2020
AuthorsFengjiang Liu / Jingxi Liang / Bing Zhang / Yan Gao / Xiuna Yang / Tianyu Hu / Haitao Yang / Wenqing Xu / Luke W Guddat / Zihe Rao /
PubMed AbstractIn bacteria, adenosine 5'-triphosphate (ATP)-binding cassette (ABC) importers are essential for the uptake of nutrients including the nonreducing disaccharide trehalose, a metabolite that is crucial ...In bacteria, adenosine 5'-triphosphate (ATP)-binding cassette (ABC) importers are essential for the uptake of nutrients including the nonreducing disaccharide trehalose, a metabolite that is crucial for the survival and virulence of several human pathogens including SugABC is an ABC transporter that translocates trehalose from the periplasmic lipoprotein LpqY into the cytoplasm of mycobacteria. Here, we report four high-resolution cryo-electron microscopy structures of the mycobacterial LpqY-SugABC complex to reveal how it binds and passes trehalose through the membrane to the cytoplasm. A unique feature observed in this system is the initial mode of capture of the trehalose at the LpqY interface. Uptake is achieved by a pivotal rotation of LpqY relative to SugABC, moving from an open and accessible conformation to a clamped conformation upon trehalose binding. These findings enrich our understanding as to how ABC transporters facilitate substrate transport across the membrane in Gram-positive bacteria.
External linksSci Adv / PubMed:33127676 / PubMed Central
MethodsEM (single particle)
Resolution3.3 - 3.78 Å
Structure data

30327

7cad

EMDB-30327, PDB-7cad:
Mycobacterium smegmatis SugABC complex
Method: EM (single particle) / Resolution: 3.41 Å

30328

7cae

EMDB-30328, PDB-7cae:
Mycobacterium smegmatis LpqY-SugABC complex in the resting state
Method: EM (single particle) / Resolution: 3.44 Å

30329

7caf

EMDB-30329, PDB-7caf:
Mycobacterium smegmatis LpqY-SugABC complex in the pre-translocation state
Method: EM (single particle) / Resolution: 3.3 Å

30330

7cag

EMDB-30330, PDB-7cag:
Mycobacterium smegmatis LpqY-SugABC complex in the catalytic intermediate state
Method: EM (single particle) / Resolution: 3.78 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM / Adenosine triphosphate

Source
  • mycolicibacterium smegmatis mc2 155 (bacteria)
  • mycolicibacterium smegmatis (strain atcc 700084 / mc(2)155) (bacteria)
KeywordsTRANSPORT PROTEIN / ABC transporter / Lipoprotein / Mycobacteria / Trehalose / Trehalose-specific importer

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