Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Structural basis of the complete poxvirus transcription initiation process.
Journal, issue, pages	Nat Struct Mol Biol, Vol. 28, Issue 10, Page 779-788, Year 2021
Publish date	Sep 23, 2021
Authors	Clemens Grimm / Julia Bartuli / Bettina Boettcher / Aladar A Szalay / Utz Fischer /
PubMed Abstract	Poxviruses express their genes in the cytoplasm of infected cells using a virus-encoded multi-subunit polymerase (vRNAP) and unique transcription factors. We present cryo-EM structures that uncover ...Poxviruses express their genes in the cytoplasm of infected cells using a virus-encoded multi-subunit polymerase (vRNAP) and unique transcription factors. We present cryo-EM structures that uncover the complete transcription initiation phase of the poxvirus vaccinia. In the pre-initiation complex, the heterodimeric early transcription factor VETFs/l adopts an arc-like shape spanning the polymerase cleft and anchoring upstream and downstream promoter elements. VETFI emerges as a TBP-like protein that inserts asymmetrically into the DNA major groove, triggers DNA melting, ensures promoter recognition and enforces transcription directionality. The helicase VETFs fosters promoter melting and the phospho-peptide domain (PPD) of vRNAP subunit Rpo30 enables transcription initiation. An unprecedented upstream promoter scrunching mechanism assisted by the helicase NPH-I probably fosters promoter escape and transition into elongation. Our structures shed light on unique mechanisms of poxviral gene expression and aid the understanding of thus far unexplained universal principles in transcription.
External links	Nat Struct Mol Biol / PubMed:34556871
Methods	EM (single particle)
Resolution	2.7 - 3.15 Å
Structure data	11824 7amv EMDB-11824, PDB-7amv: Atomic structure of the poxvirus transcription pre-initiation complex in the initially melted state Method: EM (single particle) / Resolution: 2.8 Å 11843 7aof EMDB-11843, PDB-7aof: Atomic structure of the poxvirus transcription late pre-initiation complex Method: EM (single particle) / Resolution: 2.98 Å 11844 7aoh EMDB-11844, PDB-7aoh: Atomic structure of the poxvirus late initially transcribing complex Method: EM (single particle) / Resolution: 2.7 Å 11848 7aoz EMDB-11848, PDB-7aoz: Atomic structure of the poxvirus transcription initiation complex in conformation 1 Method: EM (single particle) / Resolution: 2.85 Å 11850 7ap8 EMDB-11850, PDB-7ap8: Atomic structure of the poxvirus initially transcribing complex in conformation 2 Method: EM (single particle) / Resolution: 3.15 Å 11851 7ap9 EMDB-11851, PDB-7ap9: Atomic structure of the poxvirus initially transcribing complex in conformation 3 Method: EM (single particle) / Resolution: 3.01 Å
Chemicals	ChemComp-MG: Unknown entry ChemComp-ZN: Unknown entry
Source	vaccinia virus glv-1h68 synthetic construct (others)
Keywords	TRANSCRIPTION / Vaccinia / Virus / RNA polymerase / DNA-dependent / pre-initiation complex / PIC / initially melted / poxvirus / poxviridae / initiation complex