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TitleStructural insights into outer membrane asymmetry maintenance in Gram-negative bacteria by MlaFEDB.
Journal, issue, pagesNat Struct Mol Biol, Vol. 28, Issue 1, Page 81-91, Year 2021
Publish dateNov 16, 2020
AuthorsXiaodi Tang / Shenghai Chang / Wen Qiao / Qinghua Luo / Yuejia Chen / Zhiying Jia / James Coleman / Ke Zhang / Ting Wang / Zhibo Zhang / Changbin Zhang / Xiaofeng Zhu / Xiawei Wei / Changjiang Dong / Xing Zhang / Haohao Dong /
PubMed AbstractThe highly asymmetric outer membrane of Gram-negative bacteria functions in the defense against cytotoxic substances, such as antibiotics. The Mla pathway maintains outer membrane lipid asymmetry by ...The highly asymmetric outer membrane of Gram-negative bacteria functions in the defense against cytotoxic substances, such as antibiotics. The Mla pathway maintains outer membrane lipid asymmetry by transporting phospholipids between the inner and outer membranes. It comprises six Mla proteins, MlaFEDBCA, including the ABC transporter MlaFEDB, which functions via an unknown mechanism. Here we determine cryo-EM structures of Escherichia coli MlaFEDB in an apo state and bound to phospholipid, ADP or AMP-PNP to a resolution of 3.3-4.1 Å and establish a proteoliposome-based transport system that includes MlaFEDB, MlaC and MlaA-OmpF to monitor the transport direction of phospholipids. In vitro transport assays and in vivo membrane permeability assays combined with mutagenesis identify functional residues that not only recognize and transport phospholipids but also regulate the activity and structural stability of the MlaFEDB complex. Our results provide mechanistic insights into the Mla pathway, which could aid antimicrobial drug development.
External linksNat Struct Mol Biol / PubMed:33199922
MethodsEM (single particle)
Resolution3.3 - 4.1 Å
Structure data

11547

6zy2

EMDB-11547, PDB-6zy2:
Cryo-EM structure of apo MlaFEDB
Method: EM (single particle) / Resolution: 3.6 Å

11548

6zy3

EMDB-11548, PDB-6zy3:
Cryo-EM structure of MlaFEDB in complex with phospholipid
Method: EM (single particle) / Resolution: 3.3 Å

11549

6zy4

EMDB-11549, PDB-6zy4:
Cryo-EM structure of MlaFEDB in complex with ADP
Method: EM (single particle) / Resolution: 4.1 Å

11555

6zy9

EMDB-11555, PDB-6zy9:
Cryo-EM structure of MlaFEDB in complex with AMP-PNP
Method: EM (single particle) / Resolution: 3.3 Å

Chemicals

ChemComp-PEE:
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine / DOPE, phospholipid*YM

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM

ChemComp-MG:
Unknown entry

ChemComp-ANP:
PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / AMP-PNP, energy-carrying molecule analogue*YM

Source
  • escherichia coli b185 (bacteria)
  • escherichia coli (E. coli)
  • escherichia coli 909945-2 (bacteria)
  • escherichia coli 2.3916 (bacteria)
KeywordsLIPID TRANSPORT / phospholipid / phospholipid transport / ABC transporter / MlaFEDB / MlaFE / MlaD / MlaE / MlaF / MlaB / outer membrane / Mla transport pathway

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