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-Structure paper
Title | Structural basis of mitochondrial translation. |
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Journal, issue, pages | Elife, Vol. 9, Year 2020 |
Publish date | Aug 19, 2020 |
Authors | Shintaro Aibara / Vivek Singh / Angelika Modelska / Alexey Amunts / |
PubMed Abstract | Translation of mitochondrial messenger RNA (mt-mRNA) is performed by distinct mitoribosomes comprising at least 36 mitochondria-specific proteins. How these mitoribosomal proteins assist in the ...Translation of mitochondrial messenger RNA (mt-mRNA) is performed by distinct mitoribosomes comprising at least 36 mitochondria-specific proteins. How these mitoribosomal proteins assist in the binding of mt-mRNA and to what extent they are involved in the translocation of transfer RNA (mt-tRNA) is unclear. To visualize the process of translation in human mitochondria, we report ~3.0 Å resolution structure of the human mitoribosome, including the L7/L12 stalk, and eight structures of its functional complexes with mt-mRNA, mt-tRNAs, recycling factor and additional trans factors. The study reveals a transacting protein module LRPPRC-SLIRP that delivers mt-mRNA to the mitoribosomal small subunit through a dedicated platform formed by the mitochondria-specific protein mS39. Mitoribosomal proteins of the large subunit mL40, mL48, and mL64 coordinate translocation of mt-tRNA. The comparison between those structures shows dynamic interactions between the mitoribosome and its ligands, suggesting a sequential mechanism of conformational changes. |
External links | Elife / PubMed:32812867 / PubMed Central |
Methods | EM (single particle) |
Resolution | 3.5 - 5.0 Å |
Structure data | EMDB-11390, PDB-6zs9: EMDB-11391, PDB-6zsa: EMDB-11392, PDB-6zsb: EMDB-11393, PDB-6zsc: EMDB-11394, PDB-6zsd: EMDB-11395, PDB-6zse: EMDB-11397, PDB-6zsg: EMDB-12877, PDB-7og4: |
Chemicals | ChemComp-ZN: ChemComp-MG: ChemComp-DOL: ChemComp-GTP: ChemComp-H8Q: |
Source |
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Keywords | TRANSLATION / Mitochondria / Ribosome / mRNA / tRNA |