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TitleThe molecular basis for recognition of 5'-NNNCC-3' PAM and its methylation state by Acidothermus cellulolyticus Cas9.
Journal, issue, pagesNat Commun, Vol. 11, Page 6346-6346, Year 2020
Publish dateMar 27, 2020 (structure data deposition date)
AuthorsDas, A. / Hand, T.H. / Smith, C.L. / Wickline, E. / Zawrotny, M. / Li, H.
External linksNat Commun / PubMed:33311465
MethodsX-ray diffraction
Resolution2.91 - 3.61 Å
Structure data

PDB-6wbr:
Crystal structure of AceCas9 bound with guide RNA and DNA with 5'-NNNCC-3' PAM
Method: X-RAY DIFFRACTION / Resolution: 2.91 Å

PDB-6wc0:
Crystal structure of AceCas9 bound with guide RNA and DNA with 5'-NNNTC-3' PAM
Method: X-RAY DIFFRACTION / Resolution: 3.61 Å

Source
  • acidothermus cellulolyticus (strain atcc 43068 / 11b) (bacteria)
  • acidothermus cellulolyticus 11b (bacteria)
  • synthetic construct (others)
KeywordsRNA BINDING PROTEIN/DNA/RNA / DNA endonuclease / CRISPR-Cas9 / HNH / RuvC / RNA BINDING PROTEIN / RNA BINDING PROTEIN-DNA-RNA complex / RNA BINDING PROTEIN/RNA/DNA / RNA BINDING PROTEIN-RNA-DNA complex

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