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TitleStructure of the Bcs1 AAA-ATPase suggests an airlock-like translocation mechanism for folded proteins.
Journal, issue, pagesNat Struct Mol Biol, Vol. 27, Issue 2, Page 142-149, Year 2020
Publish dateJan 27, 2020
AuthorsLukas Kater / Nikola Wagener / Otto Berninghausen / Thomas Becker / Walter Neupert / Roland Beckmann /
PubMed AbstractSome proteins require completion of folding before translocation across a membrane into another cellular compartment. Yet the permeability barrier of the membrane should not be compromised and ...Some proteins require completion of folding before translocation across a membrane into another cellular compartment. Yet the permeability barrier of the membrane should not be compromised and mechanisms have remained mostly elusive. Here, we present the structure of Saccharomyces cerevisiae Bcs1, an AAA-ATPase of the inner mitochondrial membrane. Bcs1 facilitates the translocation of the Rieske protein, Rip1, which requires folding and incorporation of a 2Fe-2S cluster before translocation and subsequent integration into the bc1 complex. Surprisingly, Bcs1 assembles into exclusively heptameric homo-oligomers, with each protomer consisting of an amphipathic transmembrane helix, a middle domain and an ATPase domain. Together they form two aqueous vestibules, the first being accessible from the mitochondrial matrix and the second positioned in the inner membrane, with both separated by the seal-forming middle domain. On the basis of this unique architecture, we propose an airlock-like translocation mechanism for folded Rip1.
External linksNat Struct Mol Biol / PubMed:31988523
MethodsEM (single particle)
Resolution3.4 - 4.6 Å
Structure data

10192

6sh3

EMDB-10192, PDB-6sh3:
Structure of the ADP state of the heptameric Bcs1 AAA-ATPase
Method: EM (single particle) / Resolution: 3.4 Å

10193

6sh4

EMDB-10193: Structure of the Apo2 state of the heptameric Bcs1 AAA-ATPase (C7 and C1 symmetrized maps).
PDB-6sh4: Structure of the Apo1 state of the heptameric Bcs1 AAA-ATPase.
Method: EM (single particle) / Resolution: 4.4 Å

10194

6sh5

EMDB-10194: Structure of the Apo2 state of the heptameric Bcs1 AAA-ATPase (C7 and C1 symmetrized maps)
PDB-6sh5: Structure of the Apo2 state of the heptameric Bcs1 AAA-ATPase
Method: EM (single particle) / Resolution: 4.6 Å

Chemicals

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM

Source
  • saccharomyces cerevisiae (brewer's yeast)
KeywordsTRANSLOCASE / translocation / Rieske / mitochondira / inner mitochondiral membrane

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