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TitleTwo new ene-reductases from photosynthetic extremophiles enlarge the panel of old yellow enzymes: CtOYE and GsOYE.
Journal, issue, pagesAppl. Microbiol. Biotechnol., Vol. 104, Page 2051-2066, Year 2020
Publish dateJun 14, 2019 (structure data deposition date)
AuthorsRobescu, M.S. / Niero, M. / Hall, M. / Cendron, L. / Bergantino, E.
External linksAppl. Microbiol. Biotechnol. / PubMed:31930452
MethodsX-ray diffraction
Resolution1.35 - 2.38 Å
Structure data

PDB-6s0g:
Crystal structure of ene-reductase GsOYE from Galdieria sulphuraria
Method: X-RAY DIFFRACTION / Resolution: 1.45 Å

PDB-6s23:
Crystal structure of ene-reductase GsOYE from Galleria sulphuraria in complex with 2-methyl-cyclopenten-1-one
Method: X-RAY DIFFRACTION / Resolution: 2.38 Å

PDB-6s31:
Crystal structure of ene-reductase GsOYE from Galdieria sulphuraria in complex with 4-Hydroxybenzaldehyde
Method: X-RAY DIFFRACTION / Resolution: 1.63 Å

PDB-6s32:
Crystal structure of ene-reductase CtOYE from Chroococcidiopsis thermalis.
Method: X-RAY DIFFRACTION / Resolution: 1.35 Å

Chemicals

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-FMN:
FLAVIN MONONUCLEOTIDE

ChemComp-CL:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-KSW:
2-methylcyclopenta-2,4-dien-1-one

ChemComp-MG:
Unknown entry

ChemComp-HBA:
P-HYDROXYBENZALDEHYDE

ChemComp-ACT:
ACETATE ION

ChemComp-BEN:
BENZAMIDINE

Source
  • galdieria sulphuraria (eukaryote)
  • chroococcidiopsis thermalis (bacteria)
KeywordsFLAVOPROTEIN / ene-reductase / Old Yellow Enzyme / Biocatalysis / OXIDOREDUCTASE

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