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TitleBacteriophage MS2 displays unreported capsid variability assembling T = 4 and mixed capsids.
Journal, issue, pagesMol Microbiol, Vol. 113, Issue 1, Page 143-152, Year 2020
Publish dateNov 5, 2019
AuthorsNatàlia de Martín Garrido / Michael A Crone / Kailash Ramlaul / Paul A Simpson / Paul S Freemont / Christopher H S Aylett /
PubMed AbstractBacteriophage MS2 is a positive-sense, single-stranded RNA virus encapsulated in an asymmetric T = 3 pseudo-icosahedral capsid. It infects Escherichia coli through the F-pilus, in which it binds ...Bacteriophage MS2 is a positive-sense, single-stranded RNA virus encapsulated in an asymmetric T = 3 pseudo-icosahedral capsid. It infects Escherichia coli through the F-pilus, in which it binds through a maturation protein incorporated into its capsid. Cryogenic electron microscopy has previously shown that its genome is highly ordered within virions, and that it regulates the assembly process of the capsid. In this study, we have assembled recombinant MS2 capsids with non-genomic RNA containing the capsid incorporation sequence, and investigated the structures formed, revealing that T = 3, T = 4 and mixed capsids between these two triangulation numbers are generated, and resolving structures of T = 3 and T = 4 capsids to 4 Å and 6 Å respectively. We conclude that the basic MS2 capsid can form a mix of T = 3 and T = 4 structures, supporting a role for the ordered genome in favouring the formation of functional T = 3 virions.
External linksMol Microbiol / PubMed:31618483 / PubMed Central
MethodsEM (single particle)
Resolution3.9 - 6.0 Å
Structure data

4989

6rrs

EMDB-4989, PDB-6rrs:
T=3 MS2 Virus-like particle
Method: EM (single particle) / Resolution: 3.9 Å

4990

6rrt

EMDB-4990, PDB-6rrt:
T=4 MS2 Virus-like-particle
Method: EM (single particle) / Resolution: 6.0 Å

Source
  • escherichia phage ms2 (virus)
KeywordsVIRUS LIKE PARTICLE / MS2 / T=3 / BACTERIOPHAGE / VLP / T=4

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