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Structure paper

TitleX-ray-induced photoreduction of heme metal centers rapidly induces active-site perturbations in a protein-independent manner.
Journal, issue, pagesJ. Biol. Chem., Vol. 295, Page 13488-13501, Year 2020
Publish dateMay 14, 2019 (structure data deposition date)
AuthorsPfanzagl, V. / Beale, J.H. / Michlits, H. / Schmidt, D. / Gabler, T. / Obinger, C. / Djinovic-Carugo, K. / Hofbauer, S.
External linksJ. Biol. Chem. / PubMed:32723869
MethodsX-ray diffraction
Resolution1.52 - 1.9 Å
Structure data

PDB-6rpd:
Structure of ferrous KpDyP in complex with cyanide
Method: X-RAY DIFFRACTION / Resolution: 1.52 Å

PDB-6rpe:
Structure of 5% reduced KpDyP in complex with cyanide
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

PDB-6rqy:
Structure of % reduced KpDyP
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-6rr1:
Structure of 10% reduced KpDyP
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-6rr4:
Structure of 25% reduced KpDyP
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-6rr5:
Structure of 50% reduced KpDyP
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-6rr6:
Structure of 100% reduced KpDyP
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-6rr8:
Structure of 100% reduced KpDyP (final wedges)
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

Chemicals

ChemComp-HEM:
PROTOPORPHYRIN IX CONTAINING FE

ChemComp-GOL:
GLYCEROL

ChemComp-MG:
Unknown entry

ChemComp-CYN:
CYANIDE ION

ChemComp-HOH:
WATER

Source
  • klebsiella pneumoniae (bacteria)
KeywordsOXIDOREDUCTASE / heme protein

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