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Structure paper

TitleStructure and conformational plasticity of the intact V/A-type ATPase.
Journal, issue, pagesScience, Vol. 365, Issue 6455, Year 2019
Publish dateAug 23, 2019
AuthorsLong Zhou / Leonid A Sazanov /
PubMed AbstractV (vacuolar)/A (archaeal)-type adenosine triphosphatases (ATPases), found in archaea and eubacteria, couple ATP hydrolysis or synthesis to proton translocation across the plasma membrane using the ...V (vacuolar)/A (archaeal)-type adenosine triphosphatases (ATPases), found in archaea and eubacteria, couple ATP hydrolysis or synthesis to proton translocation across the plasma membrane using the rotary-catalysis mechanism. They belong to the V-type ATPase family, which differs from the mitochondrial/chloroplast F-type ATP synthases in overall architecture. We solved cryo-electron microscopy structures of the intact V/A-ATPase, reconstituted into lipid nanodiscs, in three rotational states and two substates. These structures indicate substantial flexibility between V and V in a working enzyme, which results from mechanical competition between central shaft rotation and resistance from the peripheral stalks. We also describe details of adenosine diphosphate inhibition release, V-V torque transmission, and proton translocation, which are relevant for the entire V-type ATPase family.
External linksScience / PubMed:31439765
MethodsEM (single particle)
Resolution3.25 - 4.3 Å
Structure data

4640

6qum

EMDB-4640, PDB-6qum:
Thermus thermophilus V/A-type ATPase/synthase, rotational state 1
Method: EM (single particle) / Resolution: 3.25 Å

4699

6r0w

EMDB-4699, PDB-6r0w:
Thermus thermophilus V/A-type ATPase/synthase, rotational state 2
Method: EM (single particle) / Resolution: 3.6 Å

4700

6r0y

EMDB-4700, PDB-6r0y:
Thermus thermophilus V/A-type ATPase/synthase, rotational state 3
Method: EM (single particle) / Resolution: 3.9 Å

4702

6r0z

EMDB-4702, PDB-6r0z:
Thermus thermophilus V/A-type ATPase/synthase, rotational state 1L
Method: EM (single particle) / Resolution: 3.8 Å

4703

6r10

EMDB-4703, PDB-6r10:
Thermus thermophilus V/A-type ATPase/synthase, rotational state 1R
Method: EM (single particle) / Resolution: 4.3 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM

Source
  • Thermus thermophilus HB8 (bacteria)
  • thermus thermophilus (strain hb8 / atcc 27634 / dsm 579) (bacteria)
KeywordsMEMBRANE PROTEIN / ATP hydrolysis/synthesis / proton translocation / rotary catalysis

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