Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Mechanism of gating and partial agonist action in the glycine receptor.
Journal, issue, pages	Cell, Vol. 184, Issue 4, Page 957-968.e21, Year 2021
Publish date	Feb 18, 2021
Authors	Jie Yu / Hongtao Zhu / Remigijus Lape / Timo Greiner / Juan Du / Wei Lü / Lucia Sivilotti / Eric Gouaux /
PubMed Abstract	Ligand-gated ion channels mediate signal transduction at chemical synapses and transition between resting, open, and desensitized states in response to neurotransmitter binding. Neurotransmitters ...Ligand-gated ion channels mediate signal transduction at chemical synapses and transition between resting, open, and desensitized states in response to neurotransmitter binding. Neurotransmitters that produce maximum open channel probabilities (Po) are full agonists, whereas those that yield lower than maximum Po are partial agonists. Cys-loop receptors are an important class of neurotransmitter receptors, yet a structure-based understanding of the mechanism of partial agonist action has proven elusive. Here, we study the glycine receptor with the full agonist glycine and the partial agonists taurine and γ-amino butyric acid (GABA). We use electrophysiology to show how partial agonists populate agonist-bound, closed channel states and cryo-EM reconstructions to illuminate the structures of intermediate, pre-open states, providing insights into previously unseen conformational states along the receptor reaction pathway. We further correlate agonist-induced conformational changes to Po across members of the receptor family, providing a hypothetical mechanism for partial and full agonist action at Cys-loop receptors.
External links	Cell / PubMed:33567265 / PubMed Central
Methods	EM (single particle)
Resolution	2.9 - 4.0 Å
Structure data	20370 6plo EMDB-20370, PDB-6plo: CryoEM structure of zebra fish alpha-1 glycine receptor YGF mutant bound with GABA in SMA,open state Method: EM (single particle) / Resolution: 3.3 Å 20371 6plp EMDB-20371, PDB-6plp: CryoEM structure of zebra fish alpha-1 glycine receptor YGF mutant bound with GABA in SMA, desensitized state Method: EM (single particle) / Resolution: 3.3 Å 20372 6plq EMDB-20372, PDB-6plq: CryoEM structure of zebra fish alpha-1 glycine receptor YGF mutant bound with GABA in SMA, super-open state Method: EM (single particle) / Resolution: 3.4 Å 20373 6plr EMDB-20373, PDB-6plr: CryoEM structure of zebra fish alpha-1 glycine receptor bound with glycine in nanodisc, desensitized state Method: EM (single particle) / Resolution: 3.2 Å 20374 6pls EMDB-20374, PDB-6pls: CryoEM structure of zebra fish alpha-1 glycine receptor bound with taurine in nanodisc, desensitized state Method: EM (single particle) / Resolution: 3.0 Å 20375 6plt EMDB-20375, PDB-6plt: CryoEM structure of zebra fish alpha-1 glycine receptor bound with taurine in nanodisc, closed state Method: EM (single particle) / Resolution: 3.2 Å 20376 6plu EMDB-20376, PDB-6plu: CryoEM structure of zebra fish alpha-1 glycine receptor bound with GABA in nanodisc, desensitized state Method: EM (single particle) / Resolution: 3.3 Å 20377 6plv EMDB-20377, PDB-6plv: CryoEM structure of zebra fish alpha-1 glycine receptor bound with GABA in nanodisc, closed state Method: EM (single particle) / Resolution: 3.3 Å 20378 6plw EMDB-20378, PDB-6plw: CryoEM structure of zebra fish alpha-1 glycine receptor bound with GABA in SMA, super-open state Method: EM (single particle) / Resolution: 3.0 Å 20379 6plx EMDB-20379, PDB-6plx: CryoEM structure of zebra fish alpha-1 glycine receptor bound with GABA in SMA, desensitized state Method: EM (single particle) / Resolution: 2.9 Å 20380 6ply EMDB-20380, PDB-6ply: CryoEM structure of zebra fish alpha-1 glycine receptor bound with GABA in SMA, open state Method: EM (single particle) / Resolution: 2.9 Å 20381 6plz EMDB-20381, PDB-6plz: CryoEM structure of zebra fish alpha-1 glycine receptor bound with GABA in SMA, closed state Method: EM (single particle) / Resolution: 3.0 Å 20382 6pm0 EMDB-20382, PDB-6pm0: CryoEM structure of zebra fish alpha-1 glycine receptor bound with Taurine in SMA, super-open state Method: EM (single particle) / Resolution: 3.1 Å 20383 6pm1 EMDB-20383, PDB-6pm1: CryoEM structure of zebra fish alpha-1 glycine receptor bound with Taurine in SMA, desensitized state Method: EM (single particle) / Resolution: 3.0 Å 20384 6pm2 EMDB-20384, PDB-6pm2: CryoEM structure of zebra fish alpha-1 glycine receptor bound with Taurine in SMA, open state Method: EM (single particle) / Resolution: 3.0 Å 20385 6pm3 EMDB-20385, PDB-6pm3: CryoEM structure of zebra fish alpha-1 glycine receptor bound with Taurine in SMA, closed state Method: EM (single particle) / Resolution: 3.0 Å 20386 6pm4 EMDB-20386, PDB-6pm4: CryoEM structure of zebra fish alpha-1 glycine receptor bound with Glycine in SMA, super-open state Method: EM (single particle) / Resolution: 4.0 Å 20388 6pm5 EMDB-20388, PDB-6pm5: CryoEM structure of zebra fish alpha-1 glycine receptor bound with Glycine in SMA, desensitized state Method: EM (single particle) / Resolution: 3.1 Å 20389 6pm6 EMDB-20389, PDB-6pm6: CryoEM structure of zebra fish alpha-1 glycine receptor bound with Glycine in SMA, open state Method: EM (single particle) / Resolution: 2.9 Å 20518 6pxd EMDB-20518, PDB-6pxd: CryoEM structure of zebra fish alpha-1 glycine receptor, Apo state Method: EM (single particle) / Resolution: 2.9 Å
Chemicals	ChemComp-ABU: GAMMA-AMINO-BUTANOIC ACID / neurotransmitter, inhibitorYM / Γ-Aminobutyric acid ChemComp, No image ChemComp-UNL: Unknown ligand ChemComp-GLY: GLYCINE / Glycine ChemComp-OCT: N-OCTANE / Octane ChemComp-TAU: 2-AMINOETHANESULFONIC ACID / Taurine ChemComp-CL: Unknown entry / Chloride ChemComp-HOH*: WATER / Water
Source	danio rerio (zebrafish)
Keywords	MEMBRANE PROTEIN / glycine receptor / SMA / CryoEM / nanodisc