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Structure paper

TitleStaphylopine and pseudopaline dehydrogenase from bacterial pathogens catalyze reversible reactions and produce stereospecific metallophores.
Journal, issue, pagesJ. Biol. Chem., Vol. 294, Page 17988-18001, Year 2019
Publish dateJun 14, 2019 (structure data deposition date)
AuthorsMcFarlane, J.S. / Zhang, J. / Wang, S. / Lei, X. / Moran, G.R. / Lamb, A.L.
External linksJ. Biol. Chem. / PubMed:31615895
MethodsX-ray diffraction
Resolution1.57 - 2.18 Å
Structure data

PDB-6pbm:
Pseudopaline Dehydrogenase with NADP+ bound
Method: X-RAY DIFFRACTION / Resolution: 1.57 Å

PDB-6pbn:
Pseudopaline Dehydrogenase with (R)-Pseudopaline Soaked 1 hour
Method: X-RAY DIFFRACTION / Resolution: 1.65 Å

PDB-6pbp:
Pseudopaline Dehydrogenase with (S)-Pseudopaline Soaked 1 hour
Method: X-RAY DIFFRACTION / Resolution: 1.64 Å

PDB-6pbt:
Pseudopaline Dehydrogenase with (R)-Pseudopaline Soaked 2 hours
Method: X-RAY DIFFRACTION / Resolution: 2.18 Å

Chemicals

ChemComp-NAP:
NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-HOH:
WATER

ChemComp-AKG:
2-OXOGLUTARIC ACID

ChemComp-O77:
N-[(3S)-3-amino-3-carboxypropyl]-L-histidine

ChemComp-O7J:
N-[(1S)-1-carboxy-3-{[(1S)-1-carboxy-2-(1H-imidazol-5-yl)ethyl]amino}propyl]-L-glutamic acid

Source
  • pseudomonas aeruginosa (strain atcc 15692 / dsm 22644 / cip 104116 / jcm 14847 / lmg 12228 / 1c / prs 101 / pao1) (bacteria)
Keywordsoxidoreductase / biosynthetic protein / Opine Metallophore Dehydrogenase Enzyme

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