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TitlePathogenic siderophore ABC importer YbtPQ adopts a surprising fold of exporter.
Journal, issue, pagesSci Adv, Vol. 6, Issue 6, Page eaay7997, Year 2020
Publish dateFeb 5, 2020
AuthorsZhiming Wang / Wenxin Hu / Hongjin Zheng /
PubMed AbstractTo fight for essential metal ions, human pathogens secrete virulence-associated siderophores and retake the metal-chelated siderophores through a subfamily of adenosine triphosphate (ATP)-binding ...To fight for essential metal ions, human pathogens secrete virulence-associated siderophores and retake the metal-chelated siderophores through a subfamily of adenosine triphosphate (ATP)-binding cassette (ABC) importer, whose molecular mechanisms are completely unknown. We have determined multiple structures of the yersiniabactin importer YbtPQ from uropathogenic (UPEC) at inward-open conformation in both and substrate-bound states by cryo-electron microscopy. YbtPQ does not adopt any known fold of ABC importers but surprisingly adopts the fold of type IV ABC exporters. To our knowledge, it is the first time an exporter fold of ABC importer has been reported. We have also observed two unique features in YbtPQ: unwinding of a transmembrane helix in YbtP upon substrate release and tightly associated nucleotide-binding domains without bound nucleotides. Together, our study suggests that siderophore ABC importers have a distinct transport mechanism and should be classified as a separate subfamily of ABC importers.
External linksSci Adv / PubMed:32076651 / PubMed Central
MethodsEM (single particle)
Resolution3.4 - 4.92 Å
Structure data

20262

6p6i

EMDB-20262, PDB-6p6i:
Structure of YbtPQ importer
Method: EM (single particle) / Resolution: 3.67 Å

EMDB-20263:
Structure of YbtPQ importer in nanodisc
Method: EM (single particle) / Resolution: 4.92 Å

20264

6p6j

EMDB-20264, PDB-6p6j:
Structure of YbtPQ importer with substrate Ybt-Fe bound
Method: EM (single particle) / Resolution: 3.4 Å

Chemicals

ChemComp-O34:
yersiniabactin

ChemComp-FE:
Unknown entry

Source
  • escherichia coli (strain uti89 / upec) (bacteria)
KeywordsTRANSPORT PROTEIN / ABC importer / Yersiniabactin

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